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Requirement of Hsp90 for centrosomal function reflects its regulation of Polo kinase stability

de Cárcer, Guillermo and do Carmo Avides, Maria and Lallena, Maria José and Glover, David M. and Gonzalez, Cayetano (2001) Requirement of Hsp90 for centrosomal function reflects its regulation of Polo kinase stability. EMBO Journal, 20 (11). pp. 2878-2884. ISSN 1460-2075. PMCID PMC125474. doi:10.1093/emboj/20.11.2878.

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We have previously shown that the molecular chaperone heat shock protein 90 (Hsp90) is required to ensure proper centrosome function in Drosophila and vertebrate cells. This observation led to the hypothesis that this chaperone could be required for the stability of one or more centrosomal proteins. We have found that one of these is Polo, a protein kinase known to regulate several aspects of cell division including centrosome maturation and function. Inhibition of Hsp90 results in the inactivation of Polo kinase activity. It also leads to a loss in the ability of cytoplasmic extracts to complement the failure of salt‐stripped preparations of centrosomes to nucleate microtubules. This effect can be rescued upon addition of active recombinant Polo. We also show that Polo and Hsp90 are part of a complex and conclude that stabilization of Polo is one of the mechanisms by which Hsp90 contributes to the maintenance of functional centrosomes.

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URLURL TypeDescription CentralArticle
Glover, David M.0000-0003-0956-0103
Additional Information:© 2001 European Molecular Biology Organization. Received: 7 November 2000. Accepted: 29 March 2001. Published: 1 June 2001. We are very grateful to T.C. Kaufman, W. Whitfield, P.T. Cohen and J. Raff for valuable antibody probes and E.Hafen for the Hsp90–myc constructs. G.d.C. is supported by a postdoctoral fellowship from the Spanish ‘Ministerio de Educacion’. Work in our two laboratories is supported by the Human Capital and Mobility Programme of the EU, and by a grant from the Cancer Research Campaign of Great Britain to D.M.G.
Funding AgencyGrant Number
Ministerio de EducaciónUNSPECIFIED
European Union Regional Development FundUNSPECIFIED
Cancer Research CampaignUNSPECIFIED
Subject Keywords:centrosome; Drosophila; Hsp90; polo
Issue or Number:11
PubMed Central ID:PMC125474
Record Number:CaltechAUTHORS:20200807-170353935
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Official Citation:de Cárcer, G., do Carmo Avides, M., Lallena, M.J., Glover, D.M. and González, C. (2001), Requirement of Hsp90 for centrosomal function reflects its regulation of Polo kinase stability. The EMBO Journal, 20: 2878-2884. doi:10.1093/emboj/20.11.2878
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:104868
Deposited By: George Porter
Deposited On:25 Aug 2020 21:09
Last Modified:16 Nov 2021 18:37

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