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Cloned segment of Drosophila melanogaster rDNA containing new types of sequence insertion

Glover, David M. (1977) Cloned segment of Drosophila melanogaster rDNA containing new types of sequence insertion. Proceedings of the National Academy of Sciences of the United States of America, 74 (11). pp. 4932-4936. ISSN 0027-8424. PMCID PMC432071. doi:10.1073/pnas.74.11.4932.

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A cloned 14.3-kbase segment of Drosophila melanogaster rDNA (Dm207) is described in which only a 4-kbase region is homologous to a cloned 17-kbase rDNA repeating unit, Dm103; this 4-kbase region consists of part of the 28S rRNA gene and most but not all of the adjacent transcribed spacer that normally connects the 18S and 28S genes. The transcribed spacer in Dm207 is interrupted by a 2.2-kbase stretch of DNA that does not contain any 18S gene sequences. At the other end of the 4-kbase homology, the 28S gene is interrupted by an 8.1-kbase stretch of DNA at a position equivalent to the site of the 28S insertion found in the 17-kbase units. The question of whether the 2.2-kbase and 8.1-kbase interrupter segments in Dm207 derive from longer insertions into the transcribed spacer and 28S genes of a very long repeating unit (greater than or equal to 22 kbases) or represent a region of the chromosomal DNA into which a 4-kbase fragment of rDNA has been inserted is discussed.

Item Type:Article
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URLURL TypeDescription CentralArticle
Glover, David M.0000-0003-0956-0103
Additional Information:© 1977 National Academy of Sciences. Communicated by David S. Hogness, August 25, 1977. I thank Pete Burrows for his excellent technical assistance in some of the experiments described. This work was supported by a project grant from the Medical Research Council. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
Medical Research Council (UK)UNSPECIFIED
Subject Keywords:DNA-RNA hybridization/R-loops/heteroduplexes/restriction endonuclease sites/renaturation kinetics
Issue or Number:11
PubMed Central ID:PMC432071
Record Number:CaltechAUTHORS:20200807-170354415
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Official Citation:Cloned segment of Drosophila melanogaster rDNA containing new types of sequence insertion D M Glover Proceedings of the National Academy of Sciences Nov 1977, 74 (11) 4932-4936; DOI: 10.1073/pnas.74.11.4932
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:104872
Deposited By: George Porter
Deposited On:11 Aug 2020 14:28
Last Modified:16 Nov 2021 18:37

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