CaltechAUTHORS
  A Caltech Library Service

Local auxin biosynthesis is required for root regeneration after wounding

Matosevich, Rotem and Cohen, Itay and Gil-Yarom, Naama and Modrego, Abelardo and Friedlander-Shani, Lilach and Verna, Carla and Scarpella, Enrico and Efroni, Idan (2020) Local auxin biosynthesis is required for root regeneration after wounding. Nature Plants, 6 (8). pp. 1020-1030. ISSN 2055-0278. https://resolver.caltech.edu/CaltechAUTHORS:20200811-093658118

[img] Image (JPEG) (Extended Data Fig. 1: Expression of sensitive auxin reporters in regenerating root tip) - Supplemental Material
See Usage Policy.

400Kb
[img] Image (JPEG) (Extended Data Fig. 2: Analysis of gene expression changes in regenerating roots treated with L-Kyn) - Supplemental Material
See Usage Policy.

147Kb
[img] Image (JPEG) (Extended Data Fig. 3: Shoot-derived auxin is not required for regeneration) - Supplemental Material
See Usage Policy.

102Kb
[img] Image (JPEG) (Extended Data Fig. 4: Expression of wound-induced YUC during regeneration) - Supplemental Material
See Usage Policy.

82Kb
[img] Image (JPEG) (Extended Data Fig. 5: Strong lines of pYUC9:amirYUC develop pin-like terminated meristems) - Supplemental Material
See Usage Policy.

103Kb
[img] Image (JPEG) (Extended Data Fig. 6: Regeneration and tissue pattern recovery in auxin-transport deficient roots) - Supplemental Material
See Usage Policy.

280Kb
[img] Image (JPEG) (Extended Data Fig. 7: Root meristem growth, morphology and regeneration of high-order pin mutants) - Supplemental Material
See Usage Policy.

304Kb
[img] Image (JPEG) (Extended Data Fig. 8: Effects of disrupting auxin efflux and influx on regeneration) - Supplemental Material
See Usage Policy.

294Kb
[img] Image (JPEG) (Extended Data Fig. 9: Expression of auxin reporters in high-cut root tips) - Supplemental Material
See Usage Policy.

86Kb
[img] MS Excel (Supplementary Tables 1–3) - Supplemental Material
See Usage Policy.

200Kb
[img] PDF (Reporting Summary) - Supplemental Material
See Usage Policy.

68Kb
[img] Image (PNG) - Cover Image
See Usage Policy.

83Kb

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20200811-093658118

Abstract

The root meristem can regenerate following removal of its stem-cell niche by recruitment of remnant cells from the stump. Regeneration is initiated by rapid accumulation of auxin near the injury site but the source of this auxin is unknown. Here, we show that auxin accumulation arises from the activity of multiple auxin biosynthetic sources that are newly specified near the cut site and that their continuous activity is required for the regeneration process. Auxin synthesis is highly localized while PIN-mediated transport is dispensable for auxin accumulation and tip regeneration. Roots lacking the activity of the regeneration competence factor ERF115, or that are dissected at a zone of low regeneration potential, fail to activate local auxin sources. Remarkably, restoring auxin supply is sufficient to confer regeneration capacity to these recalcitrant tissues. We suggest that regeneration competence relies on the ability to specify new local auxin sources in a precise temporal pattern.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1038/s41477-020-0737-9DOIArticle
https://rdcu.be/b6crSPublisherFree ReadCube access
ORCID:
AuthorORCID
Gil-Yarom, Naama0000-0001-7954-3136
Verna, Carla0000-0001-5312-5470
Scarpella, Enrico0000-0003-2962-0329
Efroni, Idan0000-0002-0219-8685
Additional Information:© 2020 Springer Science and Business Media LLC. Received 15 August 2019; Accepted 02 July 2020; Published 03 August 2020. We thank J. Alonso, A. Stepanova and L. de Veylder for sharing research material, Ken-ichiro Hayashi for providing yucasin DF, M. De Martino and A. Lepar for cloning and nightly assistance, and Y. Eshed and S. Savaldi-Goldstein for comments and discussions. I.E. is supported by the Israeli Science Foundation (grant no. ISF966/17) and the Howard Hughes Medical Institute International Research Scholar Grant (grant no. 55008730). Data availability: RNA-seq data are available via GEO (series GSE145488). Author Contributions: R.M. and I.E. conceived and designed the study. R.M., I.C., N.G.Y., A.M. and I.E. carried out the experiments. L.F.S. generated the pGL2:iaaH line. C.V. and E.S. generated pin mutants. R.M. and I.E. wrote the paper. The authors declare no competing interests.
Funders:
Funding AgencyGrant Number
Israel Science FoundationISF966/17
Howard Hughes Medical Institute55008730
Subject Keywords:Auxin; Plant regeneration; Plant stem cell; Root apical meristem
Issue or Number:8
Record Number:CaltechAUTHORS:20200811-093658118
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200811-093658118
Official Citation:Matosevich, R., Cohen, I., Gil-Yarom, N. et al. Local auxin biosynthesis is required for root regeneration after wounding. Nat. Plants 6, 1020–1030 (2020). https://doi.org/10.1038/s41477-020-0737-9
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:104906
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:11 Aug 2020 17:03
Last Modified:11 Aug 2020 17:15

Repository Staff Only: item control page