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Engineered Reproductively Isolated Species Drive Reversible Population Replacement

Buchman, Anna and Shriner, Isaiah and Yang, Ting and Liu, Junru and Antoshechkin, Igor and Marshall, John M. and Perry, Michael W. and Akbari, Omar S. (2020) Engineered Reproductively Isolated Species Drive Reversible Population Replacement. . (Unpublished)

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Engineered reproductive species barriers are useful for impeding gene flow and driving desirable genes into wild populations in a reversible threshold-dependent manner. However, methods to generate synthetic barriers have not been developed in advanced eukaryotes. To overcome this challenge, we engineered SPECIES (Synthetic Postzygotic barriers Exploiting CRISPR-based Incompatibilities for Engineering Species) to generate postzygotic reproductive barriers. Using this approach, we engineer multiple reproductively isolated SPECIES and demonstrate their threshold-dependent gene drive capabilities in D. melanogaster. Given the near-universal functionality of CRISPR tools, this approach should be portable to many species, including insect disease vectors in which confinable gene drives could be of great practical utility.

Item Type:Report or Paper (Discussion Paper)
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URLURL TypeDescription Paper
Buchman, Anna0000-0002-8775-6147
Antoshechkin, Igor0000-0002-9934-3040
Akbari, Omar S.0000-0002-6853-9884
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. Posted August 10, 2020. We thank V. Kumar for help with library preparations. Sequencing was performed at the Millard and Muriel Jacobs Genetics and Genomics Laboratory at the California Institute of Technology. We thank N. Windbichler for sharing published sgRNA lines. We also thank Dr. N. Perrimon and B. Ewen-Campen for sharing published sgRNA expressing flies and plasmids. We thank J. Reinitz for sharing antibodies. This work was supported in part by funding from UCSD lab startup funds awarded to O.S.A. and a DARPA Safe Genes Program Grant (HR0011-17-2-0047) awarded to O.S.A. and J.M.M. Author Contributions: O.S.A. conceptualized the study. A.B, I.S., T.Y., J.L., I.A., J.M.M and M.E.P performed various genetic, molecular experiments, immunohistochemistry, bioinformatic, and mathematical modelling described in the study. All authors contributed to writing, analyzed the data, and approved the final manuscript. Competing Interests: O.S.A. and A.B. have a patent pending on this technology. O.S.A is co-founder and serves on the scientific advisory board of Agragene. All other authors declare no significant competing financial, professional, or personal interests that might have influenced the performance or presentation of the work described. Data and Materials Availability: RNA sequencing data is available at NCBI SRA under accession number PRJNA578541. Fly strains engineered in this study to generate SPECIES will be available at Bloomington fly stock center with stock numbers listed in Fig S1. SPECIES lines will be made available upon request.
Group:Millard and Muriel Jacobs Genetics and Genomics Laboratory
Funding AgencyGrant Number
University of California, San DiegoUNSPECIFIED
Defense Advanced Research Projects Agency (DARPA)HR0011-17-2-0047
Record Number:CaltechAUTHORS:20200811-125007941
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Official Citation:Engineered Reproductively Isolated Species Drive Reversible Population Replacement. Anna Buchman, Isaiah Shriner, Ting Yang, Junru Liu, Igor Antoshechkin, John M Marshall, Michael W Perry, Omar S Akbari. bioRxiv 2020.08.09.242982; doi:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:104911
Deposited By: Tony Diaz
Deposited On:11 Aug 2020 20:16
Last Modified:22 Oct 2020 22:03

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