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Microscopic Object Classification through Passive Motion Observations with Holographic Microscopy

Lindensmith, Christian and Nadeau, Jay L. and Bedrossian, Manuel and Sumrall, Louis and Wallace, J. Kent and Serabyn, Eugene (2020) Microscopic Object Classification through Passive Motion Observations with Holographic Microscopy. In: 2020 IEEE Aerospace Conference. IEEE , Piscataway, NJ, pp. 1-7. ISBN 9781728127347.

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Digital holographic microscopy provides the ability to observe throughout a volume that is large compared to its resolution element without the need to refocus through the volume. This capability enables simultaneous observations of large numbers of small objects within such a volume. We have constructed a microscope that can observe a volume 0.4 x 0.4 x 1.0 μm with submicrometer resolution for observation of microorganisms and minerals in liquid environments in earth and on potential planetary missions. Because environmental samples are likely to contain mixtures of inorganics and microorganisms that are of comparable sizes near the resolution limit of the instrument, discrimination of living and non-living objects may be difficult. The motion of motile organisms can be used to readily distinguish them from non-motile objects (live or inorganic), but additional methods are required to distinguish non-motile organisms and inorganic objects that are of comparable size but different composition and structure. In this paper we evaluate the use of passive motion to make this discrimination by evaluating diffusion and buoyancy characteristics of objects in the field of view.

Item Type:Book Section
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URLURL TypeDescription ItemJournal Article
Nadeau, Jay L.0000-0001-5258-0076
Wallace, J. Kent0000-0001-5299-6899
Additional Information:© 2020 IEEE. The work described in this paper was performed in part at the Jet Propulsion Laboratory, California Institute of Technology under contract with the National Aeronautics and Space Administration and in part at the California Institute of Technology under grants GBMF-4037 and GBMF-4038 from the Gordon and Betty Moore Foundation.
Funding AgencyGrant Number
Gordon and Betty Moore FoundationGBMF-4037
Gordon and Betty Moore FoundationGBMF-4038
Record Number:CaltechAUTHORS:20200831-124315038
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Official Citation:C. Lindensmith, J. L. Nadeau, M. Bedrossian, L. Sumrall, J. K. Wallace and E. Serabyn, "Microscopic Object Classification through Passive Motion Observations with Holographic Microscopy," 2020 IEEE Aerospace Conference, Big Sky, MT, USA, 2020, pp. 1-7, doi: 10.1109/AERO47225.2020.9172746
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:105172
Deposited By: Tony Diaz
Deposited On:08 Sep 2020 19:39
Last Modified:16 Nov 2021 18:40

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