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Specific recognition of the product of a transferred major histocompatibility complex gene by cytotoxic T lymphocytes

Woodward, Jerold G. and Örn, Anders and Harmon, Richard C. and Goodenow, Robert S. and Hood, Leroy and Frelinger, Jeffrey A. (1982) Specific recognition of the product of a transferred major histocompatibility complex gene by cytotoxic T lymphocytes. Proceedings of the National Academy of Sciences of the United States of America, 79 (11). pp. 3613-3617. ISSN 0027-8424.

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Mouse L cells transfected with a genomic clone containing the H-2Ld gene (8-5 cells) were shown to function as targets for H-2Ld-specific cytotoxic T lymphocytes (CTL). The CTL-mediated lysis of 8-5 cells was shown to be H-2Ld specific by the use of (i) CTL with restricted reactivity, (ii) unlabeled target inhibition, and (iii) monoclonal antibody inhibition. We also demonstrated that 8-5 cells could function as targets for antibody-plus-complement-mediated cell lysis. Specificity was confirmed by using H-2Ld-specific monoclonal antibodies. These experiments demonstrate that the gene products of a major histocompatibility complex genomic clone can be functionally expressed in a foreign cell and can mediate immunologically specific cellular interactions.

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Hood, Leroy0000-0001-7158-3678
Additional Information:© 1982 by the National Academy of Sciences. Communicated by Charles Heidelberger, March 8, 1982. We thank Drs. David Sachs and Ted Hansen for some of the monoclonal antibodies and antisera used in these studies. We thank Dr. Roger Melvold for supplying the C-H-2dm2 mice. This work was supported by National Institutes of Health Grants CA 22662 and GM 06965. J.G.W. is a recipient of a National Arthritis Foundation Postdoctoral Fellowship; R.C.H. is supported by National Research Service Award NS-07149; R.S.G. is a Senior Lievre Fellow of the California Division of the American Cancer Society; J.A.F. is the recipient of an American Cancer Society Faculty Research Award. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:DNA-mediated gene transfer; H-2Ld gene
Issue or Number:11
Record Number:CaltechAUTHORS:WOOpnas82
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:10526
Deposited By: Tony Diaz
Deposited On:15 May 2008
Last Modified:03 Mar 2020 13:01

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