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Hmx gene conservation identifies the evolutionary origin of vertebrate cranial ganglia

Papdogiannis, Vasileios and Parker, Hugo J. and Pennati, Alessandro and Patthey, Cedric and Bronner, Marianne E. and Shimeld, Sebastian M. (2020) Hmx gene conservation identifies the evolutionary origin of vertebrate cranial ganglia. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20200909-144305477

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Abstract

The evolutionary origin of vertebrates included innovations in sensory processing associated with the acquisition of a predatory lifestyle. Vertebrates perceive external stimuli through sensory systems serviced by cranial sensory ganglia (CSG) which develop from cranial placodes; however understanding the evolutionary origin of placodes and CSGs is hampered by the gulf between living lineages and difficulty in assigning homology between cell types and structures. Here we use the Hmx gene family to address this question. We show Hmx is a constitutive component of vertebrate CSG development and that Hmx in the tunicate Ciona is able to drive the differentiation program of Bipolar Tail Neurons (BTNs), cells previously thought neural crest homologs. Using Ciona and lamprey transgenesis we demonstrate that a unique, tandemly duplicated enhancer pair regulated Hmx in the stem-vertebrate lineage. Strikingly, we also show robust vertebrate Hmx enhancer function in Ciona, demonstrating that deep conservation of the upstream regulatory network spans the evolutionary origin of vertebrates. These experiments demonstrate regulatory and functional conservation between Ciona and vertebrate Hmx, and confirm BTNs as CSG homologs. Our analysis also identifies derived evolutionary changes, including a genetic basis for secondary simplicity in Ciona and unique regulatory complexity in vertebrates.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/2020.09.07.281501DOIDiscussion Paper
ORCID:
AuthorORCID
Parker, Hugo J.0000-0001-7646-2007
Patthey, Cedric0000-0002-2627-9578
Bronner, Marianne E.0000-0003-4274-1862
Shimeld, Sebastian M.0000-0003-0195-7536
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. Posted September 07, 2020. The authors have declared no competing interest.
Record Number:CaltechAUTHORS:20200909-144305477
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200909-144305477
Official Citation:Hmx gene conservation identifies the evolutionary origin of vertebrate cranial ganglia. Vasileios Papdogiannis, Hugo J. Parker, Alessandro Pennati, Cedric Patthey, Marianne E. Bronner, Sebastian M. Shimeld. bioRxiv 2020.09.07.281501; doi: https://doi.org/10.1101/2020.09.07.281501
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:105301
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:09 Sep 2020 21:55
Last Modified:09 Sep 2020 21:55

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