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Genomic constitution of an H-2:Tla variant leukemia

Shen, F. W. and Chaganti, R. S. K. and Doucette, L. A. and Litman, G. W. and Steinmetz, M. and Hood, L. and Boyse, E. A. (1984) Genomic constitution of an H-2:Tla variant leukemia. Proceedings of the National Academy of Sciences of the United States of America, 81 (20). pp. 6447-6450. ISSN 0027-8424. PMCID PMC391941. doi:10.1073/pnas.81.20.6447.

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A TL+ leukemia of a (B6 x A)F1 hybrid mouse (H-2b/H-2a) was previously subjected to immunoselection against H-2a by passage in (B6 x A.SW)F1 mice (H-2b/H-2s). A variant leukemia line was obtained that serologically lacked not only the H-2a phenotype but also the TL phenotype determined by the linked cis Tlaa allele of strain A. The H-2b phenotype and the TL phenotype of the Tlab allele of the B6 strain, which is expressed only by leukemia cells, were retained by the variant. Southern blotting with an H-2 cDNA probe that identifies restriction fragment polymorphisms distinguishing alleles of the H-2 and Tla regions of the B6 and A strains indicates that both the H-2a and Tlaa alleles are missing from the genome of this H-2a:Tlaa negative variant. Since the variant has two apparently unaltered chromosomes 17, where the H-2:Tla complex is situated, and since the intensity of bands in Southern blotting is suggestive of H-2b homozygosity, it is considered that loss of the H-2a:Tlaa haplotype by the variant was accompanied by duplication of the H-2b:Tlab haplotype. The implied change from heterozygosity to homozygosity that the variant has undergone with respect to H-2:Tla was not paralleled by a similar change at the three other loci tested, since the variant retained heterozygosity for Pep-3 (chromosome 1), Gpi-1 (chromosome 7), and Es-1 (chromosome 8).

Item Type:Article
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URLURL TypeDescription CentralArticle
Hood, L.0000-0001-7158-3678
Additional Information:© 1984 by the National Academy of Sciences. Contributed by Edward A. Boyse, June 28, 1984. This work was supported in part by Grants CA-22131, CA-20473, and CA-34775 from the National Institutes of Health. E.A.B. is American Cancer Society Research Professor of Cell Surface Immunogenetics. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
American Cancer SocietyUNSPECIFIED
Subject Keywords:immunoselection; H-2:Tla deletion; mitotic recombination
Issue or Number:20
PubMed Central ID:PMC391941
Record Number:CaltechAUTHORS:SHEpnas84
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:10531
Deposited By: Tony Diaz
Deposited On:15 May 2008
Last Modified:08 Nov 2021 21:08

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