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HIF-2α importance for migration, proliferation and self‐renewal of trunk neural crest cells

Niklasson, Camilla U. and Fredlund, Elina and Monni, Emanuela and Lindvall, Jessica M. and Kokaia, Zaal and Hammarlund, Emma U. and Bronner, Marianne E. and Mohlin, Sofie (2020) HIF-2α importance for migration, proliferation and self‐renewal of trunk neural crest cells. Developmental Dynamics . ISSN 1058-8388. (In Press) https://resolver.caltech.edu/CaltechAUTHORS:20200923-074659440

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Abstract

Background: The neural crest is a transient embryonic stem cell population. Hypoxia Inducible Factor (HIF)‐2α is associated with neural crest stem cell appearance and aggressiveness in tumors. However, little is known about its role in normal neural crest development. Results: Here we show that HIF‐2α is expressed in trunk neural crest cells of human, murine and avian embryos. Knockdown as well as overexpression of HIF‐2α in vivo causes developmental delays, induces proliferation and self‐renewal capacity of neural crest cells while decreasing the proportion of neural crest cells that migrate ventrally to sympathoadrenal sites. Reflecting the in vivo phenotype, transcriptome changes after loss of HIF‐2α reveal enrichment of genes associated with cancer, invasion, epithelial‐to‐mesenchymal transition and growth arrest. Conclusions: Taken together, these results suggest that expression levels of HIF‐2α must be strictly controlled during normal trunk neural crest development and that dysregulated levels affects several important features connected to stemness, migration and development.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/dvdy.253DOIArticle
ORCID:
AuthorORCID
Bronner, Marianne E.0000-0003-4274-1862
Mohlin, Sofie0000-0002-2458-3963
Additional Information:© 2020 Wiley Periodicals, Inc. Accepted manuscript online: 17 September 2020; Manuscript accepted: 11 September 2020; Manuscript revised: 02 September 2020; Manuscript received: 29 July 2020.
Subject Keywords:Neural crest; Trunk neural crest; HIF‐2α; embryogenesis; migration; stem cells
Record Number:CaltechAUTHORS:20200923-074659440
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200923-074659440
Official Citation:Niklasson, C.U., Fredlund, E., Monni, E., Lindvall, J.M., Kokaia, Z., Hammarlund, E.U., Bronner, M.E. and Mohlin, S. (2020), HIF‐2α importance for migration, proliferation and self‐renewal of trunk neural crest cells. Developmental Dynamics. Accepted Author Manuscript. doi:10.1002/dvdy.253
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:105484
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:23 Sep 2020 14:59
Last Modified:23 Sep 2020 14:59

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