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polo, a mitotic mutant of Drosophila displaying abnormal spindle poles

Sunkel, Claudio E. and Glover, David M. (1988) polo, a mitotic mutant of Drosophila displaying abnormal spindle poles. Journal of Cell Science, 89 (1). pp. 25-38. ISSN 0021-9533. https://resolver.caltech.edu/CaltechAUTHORS:20200928-150331059

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Abstract

Neuroblast cells in larvae homozygous for mutant alleles of the locus polo show a high frequency of metaphases in which the chromosomes have a circular arrangement, and anaphase figures in which chromosomes appear to be randomly oriented with respect to at least one of the spindle poles. These defects appear to lead to the production of polyploid cells. Sex chromosome disjunction is affected in male meiosis, primarily in the second division, and the meiotic spindles of living cells are abnormal. One allele is a larval lethal, whereas another is semi-lethal with about 7% of homozygotes surviving as adults. Embryos from homozygous polo females have aberrant mitotic spindles that are highly branched and have broad poles. Immunofluorescence studies with an antibody that recognizes an antigen associated with the centrosome indicate that the organization of this organelle is disrupted in the mutant embryos.


Item Type:Article
Related URLs:
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https://jcs.biologists.org/content/89/1/25.longPublisherArticle
ORCID:
AuthorORCID
Glover, David M.0000-0003-0956-0103
Additional Information:© 1988 by Company of Biologists. (Received 13 August 1987 -Accepted 18 September 1987) We are most grateful to the Cancer Research Campaign for supporting this work and for a Career Development Award to D.M.G. We thank Christianne Nusslein-Volhard for allowing us to screen her collection of third chromosome maternal effect mutants from which we isolated the polo¹ allele. We are extremely grateful to Roger Karess and Alan Cheshire for their efforts in establishing a collection of dysgenic third chromosome mutants in this laboratory from which we isloated polo². We thank Cayetano Gonzalez for teaching us about male meiosis, for improving our brain-squashing techniques, and together with Pedro Ripoll for communicating results prior to publication. Many colleagues struggled through the draft manuscript and we thank them for their helpful criticisms.
Funders:
Funding AgencyGrant Number
Cancer Research CampaignUNSPECIFIED
Subject Keywords:Drosophila, mitotic mutant, spindle poles
Issue or Number:1
Record Number:CaltechAUTHORS:20200928-150331059
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200928-150331059
Official Citation:polo, a mitotic mutant of Drosophila displaying abnormal spindle poles C.E. Sunkel, D.M. Glover Journal of Cell Science 1988 89: 25-38
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:105597
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:28 Sep 2020 22:43
Last Modified:28 Sep 2020 22:43

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