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The distribution of a 'mitosis-specific' antigen during Drosophila development

Millar, Sarah E. and Freeman, Matthew and Glover, David M. (1987) The distribution of a 'mitosis-specific' antigen during Drosophila development. Journal of Cell Science, 87 (1). pp. 95-104. ISSN 0021-9533. https://resolver.caltech.edu/CaltechAUTHORS:20200928-150413759

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Abstract

We have used MPM-2, a monoclonal antibody raised against mitotic HeLa cells, to stain a Drosophila cell line, whole mounts of Drosophila embryos, and sectioned tissue from embryonic and larval stages of development. MPM-2 recognizes a major phosphoprotein of approximately 125 X 10³ M_r in Drosophila tissue culture cells that, like the mammalian MPM-2 antigen, appears to be recognized only in mitotic cells. During early embryogenesis, when the embryonic nuclei divide as a syncytium with a very short nuclear division time, MPM-2 antigen is observed within the spindle compartment at all stages of the nuclear division cycle. Upon cellularization of the embryo and lengthening of the duration of the cycle, the antigen is predominantly seen in mitotic cells. Drosophila larvae contain both diploid and polytene tissues: in diploid tissue MPM-2 staining is specifically observed over mitotic cells, as expected from its distribution in cellularized embryos. Surprisingly, antigen is also detected in the nuclei of polytene cells that replicate their DNA but do not undergo mitosis.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://jcs.biologists.org/content/87/1/95.longPublisherArticle
ORCID:
AuthorORCID
Glover, David M.0000-0003-0956-0103
Additional Information:© 1987 by Company of Biologists. (Received 28 July 1986 - Accepted 3 October 1986) We thank the Cancer Research Campaign for supporting this work and for a career development award to D.M.G.; S.E.M. was supported by a research studentship from the Medical Research Council and by a Cancer Research Campaign Bursary; M.F. was supported by a research studentship from the Medical Research Council. We also thank Dr Potu Rao for providing MPM-2 antibody, Dr Michael Ormorod for antibody against bromouridine and Dr Cornelia Gorman for P19 cells.
Funders:
Funding AgencyGrant Number
Cancer Research CampaignUNSPECIFIED
Medical Research Council (UK)UNSPECIFIED
Subject Keywords:mitosis, Drosophila, development
Issue or Number:1
Record Number:CaltechAUTHORS:20200928-150413759
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200928-150413759
Official Citation:The distribution of a ‘mitosis-specific’ antigen during Drosophila development S.E. Millar, M. Freeman, D.M. Glover Journal of Cell Science 1987 87: 95-104
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:105598
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:28 Sep 2020 22:36
Last Modified:28 Sep 2020 22:36

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