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PCNA binding proteins in Drosophila melanogaster : the analysis of a conserved PCNA binding domain

Warbrick, Emma and Heatherington, Wayne and Lane, David P. and Glover, David M. (1998) PCNA binding proteins in Drosophila melanogaster : the analysis of a conserved PCNA binding domain. Nucleic Acids Research, 26 (17). pp. 3925-3932. ISSN 1362-4962. PMCID PMC147798. doi:10.1093/nar/26.17.3925.

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The eukaryotic polymerase processivity factor, PCNA, interacts with cell cycle regulatory proteins such as p21^(WAF1/Cip1) and Gadd45, as well as with proteins involved in the mechanics of DNA repair and replication. A conserved PCNA-binding motif is found in a subset of PCNA-interacting proteins, including p21, suggesting that the regulation of these interactions is important for the co-ordination of DNA replication and repair. We have identified several classes of protein which bind to Drosophila PCNA. Two of these proteins contain the consensus PCNA-binding domain: one is the Dacapo protein, a Drosophila homologue of p21^(WAF1/Cip1), and the second is the transposase encoded by the Pogo DNA transposon. A conserved PCNA-binding domain is also present in a human relative of Pogo, named Tigger, suggesting that this domain has a functional role in this class of transposable element. This raises interesting possibilities for a novel method of transposition in which the transposase might be targeted to replicating DNA. Finally, we have investigated the use of this conserved PCNAbinding domain as a predictor of PCNA-binding capacity.

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URLURL TypeDescription CentralArticle
Glover, David M.0000-0003-0956-0103
Additional Information:© 1998 Oxford University Press. Received June 1, 1998; Revised and Accepted July 6, 1998. We thank Steve Elledge for the two-hybrid constructs and library, Daryl Henderson for Drosophila PCNA cDNA and for anti-Drosophila PCNA antibodies, Nickolai Zhelev for purified human PCNA, Kathryn Ball for peptides, Nicole Gnadt for communicating results prior to publication and Álvaro Tavares for Drosophila embryonic extracts and helpful advice. This work was supported by grants from the Cancer Research Campaign and the Association for International Cancer Research.
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Cancer Research CampaignUNSPECIFIED
Association for International Cancer ResearchUNSPECIFIED
Issue or Number:17
PubMed Central ID:PMC147798
Record Number:CaltechAUTHORS:20200928-150626644
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Official Citation:Emma Warbrick, David P. Lane, David M. Glover, Wayne Heatherington, PCNA binding proteins in Drosophila melanogaster: the analysis of a conserved PCNA binding domain, Nucleic Acids Research, Volume 26, Issue 17, 1 September 1998, Pages 3925–3932,
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:105615
Deposited By: George Porter
Deposited On:29 Sep 2020 16:03
Last Modified:16 Nov 2021 18:44

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