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Mutation of a gene for a Drosophila kinesin-like protein, Klp38B, leads to failure of cytokinesis

Ohkura, Hiroyuki and Tőrők, Tibor and Tick, Gabriella and Hoheisel, Jörg and Kiss, István and Glover, David M. (1997) Mutation of a gene for a Drosophila kinesin-like protein, Klp38B, leads to failure of cytokinesis. Journal of Cell Science, 110 (8). pp. 945-954. ISSN 0021-9533. https://resolver.caltech.edu/CaltechAUTHORS:20200930-144643398

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Abstract

Mutations in a gene (Klp38B) encoding a novel kinesin-like protein in Drosophila melanogaster lead to the formation of polyploid cells in the larval central nervous system and in the follicle cells of adult egg chambers. Some homozygous mutants survive to adulthood and also exhibit morphological defects indicative of abnormal cell cycle progression, including rough eyes, missing bristles, and abnormal abdominal cuticles. In larval brains, there is no accumulation of mitotic cells and the frequency of anaphase figures is comparable to wild type, suggesting that nuclear division is not affected. Such brains contain polyploid cells with metaphase and anaphase chromosomes associated with bipolar spindles. Such spindles have a number of unseparated centrosomes at their poles reflecting the degree of polyploidy of the cell. Follicle cells frequently contain two nuclei of roughly equal size. Taken together, we conclude that these Klp38B mutations lead to a failure of cytokinesis resulting in polyploidy, and discuss whether or not this is a direct effect of the mutation.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://jcs.biologists.org/content/110/8/945.longPublisherArticle
ORCID:
AuthorORCID
Glover, David M.0000-0003-0956-0103
Additional Information:© 1997 by Company of Biologists. (Received 6 September 1996 – Accepted, in revised form, 4 February 1997) This work was supported by grants from the Cancer Research Campaign to Dundee, and the European Union Human Capital and Mobility and PECO Programmes to Dundee and Szeged. I.K. is also grateful for support from the National Foundation for Scientific Research in Hungary (OTKA no. 924). We thank Pedro Ripoll, Luke Alphey and Yoshihiro Inoue for discussions and communication of their results prior to publication. We thank Nick Brown, Inga Sidén-Kiamos, Dan Branton, and Lisa Frenz and Mike Goldberg for their respective gifts of the cDNA library, cosmid clones, anti-spectrin antibody and staged poly(A)+ RNA. We also thank Fiona Cullen for sequencing.
Funders:
Funding AgencyGrant Number
Cancer Research CampaignUNSPECIFIED
European UnionUNSPECIFIED
Hungarian Scientific Research Fund (OTKA) 924
Subject Keywords:Drosophila, Cytokinesis, Kinesin, Klp38B
Issue or Number:8
Record Number:CaltechAUTHORS:20200930-144643398
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200930-144643398
Official Citation:Mutation of a gene for a Drosophila kinesin-like protein, Klp38B, leads to failure of cytokinesis H. Ohkura, T. Torok, G. Tick, J. Hoheisel, I. Kiss, D.M. Glover Journal of Cell Science 1997 110: 945-954
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:105697
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:01 Oct 2020 14:24
Last Modified:01 Oct 2020 14:24

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