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Mutations at the asp locus of Drosophila lead to multiple free centrosomes in syncytial embryos, but restrict centrosome duplication in larval neuroblasts

Gonzalez, C. and Saunders, R. D. C. and Casal, J. and Molina, I. and Carmena, M. and Ripoll, P. and Glover, D. M. (1990) Mutations at the asp locus of Drosophila lead to multiple free centrosomes in syncytial embryos, but restrict centrosome duplication in larval neuroblasts. Journal of Cell Science, 96 (4). pp. 605-616. ISSN 0021-9533. https://resolver.caltech.edu/CaltechAUTHORS:20200930-144645081

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Abstract

Mutations at abnormal spindle result in abnormally long and wavy microtubules in the meiotic spindles of males. Some of these spindles have a single pole and take the form of unopposed hemi-spindles. Unfertilised eggs produced by homozygous asp females may have either no nuclei, or a small number of large nuclei, consistent with there also being an effect upon female meiosis. Such eggs also display free centrosomes and independent arrays of microtubules. Embryos that have this phenotype are also present among the progeny of fertilised homozygous asp females, together with embryos that undergo varying degrees of aberrant morphogenesis, developing a variety of abnormal cuticle patterns. This latter category shows asynchronous mitoses prior to cellularisation, and has abnormal arrays of spindle microtubules. Such embryos can develop large areas that are either devoid of or have a reduced number of nuclei, in which there are centrosomes that have dissociated from the mitotic spindles. Neuroblasts in the brains of homozygous asp larvae display a high mitotic index, and have condensed chromosomes aligned as if blocked at metaphase. Immunostaining reveals that many cells contain a single centrosome connected to the metaphase chromosomes by microtubules in a hemi-spindle-like structure.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://jcs.biologists.org/content/96/4/605.longPublisherArticle
ORCID:
AuthorORCID
Glover, D. M.0000-0003-0956-0103
Additional Information:© 1990 by Company of Biologists. (Received 13 March 1990 - Accepted 26 April 1990) We thank M. Suffness for his generous gift of taxol. D.M.G. thanks the Cancer Research Campaign for a career development award and research support, and the MRC and SERC for project grants. J.C., I.M., M.C. and P.R. were funded by grants from CAYCT and FFPI.
Funders:
Funding AgencyGrant Number
Cancer Research CampaignUNSPECIFIED
Medical Research Council (UK)UNSPECIFIED
Science and Engineering Research Council (SERC)UNSPECIFIED
Comisión Asesora de Investigación Científica y Técnica (CAICYT)UNSPECIFIED
Subject Keywords:hemi-spindles, mitosis, meiosis
Issue or Number:4
Record Number:CaltechAUTHORS:20200930-144645081
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200930-144645081
Official Citation:Mutations at the asp locus of Drosophila lead to multiple free centrosomes in syncytial embryos, but restrict centrosome duplication in larval neuroblasts C. Gonzalez, R.D. Saunders, J. Casal, I. Molina, M. Carmena, P. Ripoll, D.M. Glover Journal of Cell Science 1990 96: 605-616
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:105699
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:01 Oct 2020 14:32
Last Modified:01 Oct 2020 14:32

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