Maldonado-Codina, Gabriela and Glover, David M. (1992) Cyclins A and B associate with chromatin and the polar regions of spindles, respectively, and do not undergo complete degradation at anaphase in syncytial Drosophila embryos. Journal of Cell Biology, 116 (4). pp. 967-976. ISSN 0021-9525. PMCID PMC2289331. doi:10.1083/jcb.116.4.967. https://resolver.caltech.edu/CaltechAUTHORS:20200930-144714451
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Abstract
Maternally contributed cyclin A and B proteins are initially distributed uniformly throughout the syncytial Drosophila embryo. As dividing nuclei migrate to the cortex of the embryo, the A and B cyclins become concentrated in surface layers extending to depths of approximately 30-40 microns and 5-10 microns, respectively. The initiation of nuclear envelope breakdown, spindle formation, and the initial congression of the centromeric regions of the chromosomes onto the metaphase plate all take place within the surface layer occupied by cyclin B on the apical side of the blastoderm nuclei. Cyclin B is seen mainly, but not exclusively, in the vicinity of microtubules throughout the mitotic cycle. It is most conspicuous around the centrosomes. Cyclin A is present at its highest concentrations throughout the cytoplasm during the interphase periods of the blastoderm cycles, although weak punctate staining can also be detected in the nucleus. It associates with the condensing chromosomes during prophase, segregates into daughter nuclei in association with chromosomes during anaphase, to redistribute into the cytoplasm after telophase. In contrast to the cycles following cellularization, neither cyclin is completely degraded upon the metaphase-anaphase transition.
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| Additional Information: | © The Rockefeller University Press 1992. After the Initial Publication Period, RUP will grant to the public the non-exclusive right to copy, distribute, or display the Article under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode, or updates thereof. Received for publication 12 June 1991 and in revised form 8 November 1991. We thank Harry Saumweber for T47 antibody and Dr . Sufness for taxol. We would also like to thank Scott Selleck, Cayetano Gonzalez, and Will Whitfield for their comments on the manuscript; Jonathan Pines, Tony Hunter, Eric Bailly, and Michel Bomens for communicating results before publication; and Tim Hunt for his sage advice. We are grateful to the Cancer Research Campaign for supporting this work. | |||||||||
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| Issue or Number: | 4 | |||||||||
| PubMed Central ID: | PMC2289331 | |||||||||
| DOI: | 10.1083/jcb.116.4.967 | |||||||||
| Record Number: | CaltechAUTHORS:20200930-144714451 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20200930-144714451 | |||||||||
| Official Citation: | G Maldonado-Codina, D M Glover; Cyclins A and B associate with chromatin and the polar regions of spindles, respectively, and do not undergo complete degradation at anaphase in syncytial Drosophila embryos.. J Cell Biol 15 February 1992; 116 (4): 967–976. doi: https://doi.org/10.1083/jcb.116.4.967 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 105705 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | George Porter | |||||||||
| Deposited On: | 01 Oct 2020 14:29 | |||||||||
| Last Modified: | 16 Nov 2021 18:45 |
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