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The Drosophila phosphatidylinositol transfer protein encoded by vibrator is essential to maintain cleavage-furrow ingression in cytokinesis

Gatt, Melanie K. and Glover, David M. (2006) The Drosophila phosphatidylinositol transfer protein encoded by vibrator is essential to maintain cleavage-furrow ingression in cytokinesis. Journal of Cell Science, 119 (11). pp. 2225-2235. ISSN 0021-9533. https://resolver.caltech.edu/CaltechAUTHORS:20201002-140639688

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[img] Video (AVI) (Movie 1. Wild-type primary spermatocyte undergoing successful cytokinesis) - Supplemental Material
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[img] Video (AVI) (Movie 2. Primary spermatocyte of vibrator mutant in which cytokinesis was unsuccessful) - Supplemental Material
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Abstract

Cytokinesis requires the coordination of cytoskeletal and plasma membrane dynamics. A role for phosphatidylinositol lipids has been proposed for the successful completion of cytokinesis but this is still poorly characterised. Here, we show mutants of the gene vibrator, previously found to encode the Drosophila phosphatidylinositol transfer protein, produce multinucleate cells indicative of cytokinesis failure in male meiosis. Examination of fixed preparations of mutant spermatocytes showed contractile rings of anillin and actin that were of normal appearance at early stages but were larger and less well organised at later stages of cytokinesis than in wild-type cells. Time-lapse imaging revealed sequential defects in cytokinesis of vibrator spermatocytes. In cells that fail cytokinesis, central spindle formation occurred correctly, but furrow ingression was delayed and the central spindle did not become compressed to the extent seen in wild-type cells. Cells then stalled at this point before the apparent connection between the constricted cytoskeleton and the plasma membrane was lost; the furrow then underwent elastic regression. We discuss these defects in relation to multiple functions of phosphoinositol lipids in regulating actin dynamics and membrane synthesis.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1242/jcs.02933DOIArticle
http://jcs.biologists.org/cgi/content/full/119/11/2225/DC1PublisherSupporting Information
ORCID:
AuthorORCID
Glover, David M.0000-0003-0956-0103
Additional Information:© The Company of Biologists Limited 2006. Accepted February 13, 2006. Published online May 9, 2006. This work was supported by the Medical Research Council and Cancer Research UK. We would like to thank Monica Bettencourt-Dias, Paolo D'Avino, Matthew Savoian and Tetsuya Takeda for advice and comments on the manuscript, and Ed Jones, a part II student, for his involvement during the early stage of this project. We also wish to thank Chris Field for anti-Anillin antibodies, the Bloomington and Szeged Stock Centres for fly stocks.
Funders:
Funding AgencyGrant Number
Medical Research Council (UK)UNSPECIFIED
Cancer Research UKUNSPECIFIED
Subject Keywords:Drosophila, Cytokinesis, PITP, Spermatogenesis, Vibrator
Issue or Number:11
Record Number:CaltechAUTHORS:20201002-140639688
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201002-140639688
Official Citation:The Drosophila phosphatidylinositol transfer protein encoded by vibrator is essential to maintain cleavage-furrow ingression in cytokinesis Melanie K. Gatt, David M. Glover Journal of Cell Science 2006 119: 2225-2235; doi: 10.1242/jcs.02933
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:105760
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:05 Oct 2020 16:22
Last Modified:05 Oct 2020 16:30

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