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Klp67A destabilises pre-anaphase microtubules but subsequently is required to stabilise the central spindle

Gatt, Melanie K. and Savoian, Matthew S. and Riparbelli, Maria G. and Massarelli, Chiara and Callaini, Giuliano and Glover, David M. (2005) Klp67A destabilises pre-anaphase microtubules but subsequently is required to stabilise the central spindle. Journal of Cell Science, 118 (12). pp. 2671-2682. ISSN 0021-9533. doi:10.1242/jcs.02410. https://resolver.caltech.edu/CaltechAUTHORS:20201002-140639742

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[img] PDF (Fig. S1. Anaphase B spindle length determination.An example of a klp67A mutant cell during anaphase B) - Supplemental Material
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[img] PDF (Fig. S2. Distribution of Klp67A in primary spermatocytes from various genetic backgrounds) - Supplemental Material
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[img] Video (QuickTime) (Movie 1. Anaphase B in a wild-type primary spermatocyte expressing b-tubulin-EGFP.) - Supplemental Material
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[img] Video (QuickTime) (Movie 2. Anaphase B in a klp67A mutant primary spermatocyte expressing b-tubulin-EGFP) - Supplemental Material
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[img] Video (QuickTime) (Movie 3. Central spindle formation in a wild-type primary spermatocyte.See Fig. 4A and text for details) - Supplemental Material
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[img] Video (QuickTime) (Movie 4. klp67A mutants fail to form stable central spindles. During metaphase the spindle and asters are abnormally robust) - Supplemental Material
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[img] Video (QuickTime) (Movie 5. Ectopic furrows form cytoplasts in klp67A mutants. This cell corresponds to that shown in Fig. 5) - Supplemental Material
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[img] Video (QuickTime) (Movie 6. Interior central spindles can induce cleavage furrows. This cell is also depicted in Fig. 6) - Supplemental Material
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Abstract

Klp67A is a member of the Kip3 subfamily of microtubule destabilising kinesins, the loss of which results in abnormally long and stable pre-anaphase microtubules. Here we examine its role during cytokinesis in Drosophila primary spermatocytes that require the coordinated interaction of an interior and peripheral set of central spindle microtubules. In mutants anaphase B spindles elongated with normal kinetics but bent towards the cortex. Both peripheral and interior spindle microtubules then formed diminished bundles of abnormally positioned central spindle microtubules associated with the pavarotti-KLP and KLP3A motor proteins. The minus ends of these were poorly aligned as revealed by Asp protein localisation. Furrows always initiated at the sites of central spindle bundles but could be unilateral or nonequatorially positioned. Ectopic furrows were stimulated by the interior central spindle and formed only after this structure buckled and contacted the cortex. Furrows often halted and regressed as they could not be sustained by the central spindles that became increasing unstable over time and often completely degraded. Consistent with this, actin and anillin failed to form homogenous bands. Thus, the Klp67A microtubule catastrophe factor is required for cytokinesis by regulating both the formation and stability of the central spindle.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1242/jcs.02410DOIArticle
http://jcs.biologists.org/cgi/content/full/118/12/2671/DC1PublisherSupporting Information
ORCID:
AuthorORCID
Savoian, Matthew S.0000-0002-2594-3879
Callaini, Giuliano0000-0003-2252-0309
Glover, David M.0000-0003-0956-0103
Additional Information:© The Company of Biologists Limited 2005. Accepted March 22, 2005. Published online June 8, 2005. This work was made possible by a MRC grant to D.M.G. and grants from PAR (University of Sienna) to M.G.R. and G.C., respectively.
Funders:
Funding AgencyGrant Number
Medical Research Council (UK)UNSPECIFIED
University of SiennaUNSPECIFIED
Subject Keywords:Kinesin-like protein, Kip3 subfamily, Cytokinesis, Drosophila, Meiosis
Issue or Number:12
DOI:10.1242/jcs.02410
Record Number:CaltechAUTHORS:20201002-140639742
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201002-140639742
Official Citation:Klp67A destabilises pre-anaphase microtubules but subsequently is required to stabilise the central spindle Melanie K. Gatt, Matthew S. Savoian, Maria G. Riparbelli, Chiara Massarelli, Giuliano Callaini, David M. Glover Journal of Cell Science 2005 118: 2671-2682; doi: 10.1242/jcs.02410
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:105761
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:05 Oct 2020 16:34
Last Modified:16 Nov 2021 18:46

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