Lester, Henry A. and Chang, Hai Won (1977) Response of acetylcholine receptors to rapid photochemically produced increases in agonist concentration. Nature, 266 (5600). pp. 373-374. ISSN 0028-0836. doi:10.1038/266373a0. https://resolver.caltech.edu/CaltechAUTHORS:20201005-113705890
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Abstract
SYNAPTIC transmission to muscle fibres and electroplaques takes place on a millisecond timescale. To study the molecular events leading to activation of the cholinergic receptor channel, we have developed a method to produce similarly rapid, spatially uniform steps of agonist concentration near intact postsynaptic membranes. Electrophorus electroplaques in a voltage-clamp apparatus were exposed to a solution containing a photochromic compound. Initially the compound was in a predominantly cis form, which had little effect on the membrane. During a brief light flash, some of the compound was isomerised to the trans isomer, which is a cholinergic agonist. As a result the membrane conductance increased along a timecourse which reflects the rate processes underlying activation of the cholinergic receptor channel.
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| Additional Information: | © Nature Publishing Group 1977. Received 01 November 1976. Accepted 25 January 1977. We thank D. Williams for assistance with the animals, W. L. Crowder for help with electronics, D. D. Koblin for participating in some of the experiments, M. A. Raftery for the use of a spectrophotometer, E. Neumann for helpful discussion, and the Chadwick-Helmuth Corporation (Monrovia, California) for the loan of a flash tube and power supply. This work was supported by the NIH, by the Muscular Dystrophy Association, and by an Alfred P. Sloan Fellowship to H.A.L. | |||||||||
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| Issue or Number: | 5600 | |||||||||
| DOI: | 10.1038/266373a0 | |||||||||
| Record Number: | CaltechAUTHORS:20201005-113705890 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20201005-113705890 | |||||||||
| Official Citation: | LESTER, H., WON CHANG, H. Response of acetylcholine receptors to rapid photochemically produced increases in agonist concentration. Nature 266, 373–374 (1977). https://doi.org/10.1038/266373a0 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 105796 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | George Porter | |||||||||
| Deposited On: | 05 Oct 2020 19:49 | |||||||||
| Last Modified: | 16 Nov 2021 18:46 |
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