CaltechAUTHORS
  A Caltech Library Service

Response of acetylcholine receptors to rapid photochemically produced increases in agonist concentration

Lester, Henry A. and Chang, Hai Won (1977) Response of acetylcholine receptors to rapid photochemically produced increases in agonist concentration. Nature, 266 (5600). pp. 373-374. ISSN 0028-0836. https://resolver.caltech.edu/CaltechAUTHORS:20201005-113705890

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20201005-113705890

Abstract

SYNAPTIC transmission to muscle fibres and electroplaques takes place on a millisecond timescale. To study the molecular events leading to activation of the cholinergic receptor channel, we have developed a method to produce similarly rapid, spatially uniform steps of agonist concentration near intact postsynaptic membranes. Electrophorus electroplaques in a voltage-clamp apparatus were exposed to a solution containing a photochromic compound. Initially the compound was in a predominantly cis form, which had little effect on the membrane. During a brief light flash, some of the compound was isomerised to the trans isomer, which is a cholinergic agonist. As a result the membrane conductance increased along a timecourse which reflects the rate processes underlying activation of the cholinergic receptor channel.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1038/266373a0DOIArticle
https://rdcu.be/b76AWPublisherFree ReadCube access
ORCID:
AuthorORCID
Lester, Henry A.0000-0002-5470-5255
Additional Information:© Nature Publishing Group 1977. Received 01 November 1976. Accepted 25 January 1977. We thank D. Williams for assistance with the animals, W. L. Crowder for help with electronics, D. D. Koblin for participating in some of the experiments, M. A. Raftery for the use of a spectrophotometer, E. Neumann for helpful discussion, and the Chadwick-Helmuth Corporation (Monrovia, California) for the loan of a flash tube and power supply. This work was supported by the NIH, by the Muscular Dystrophy Association, and by an Alfred P. Sloan Fellowship to H.A.L.
Funders:
Funding AgencyGrant Number
NIHUNSPECIFIED
Muscular Dystrophy AssociationUNSPECIFIED
Alfred P. Sloan FoundationUNSPECIFIED
Issue or Number:5600
Record Number:CaltechAUTHORS:20201005-113705890
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201005-113705890
Official Citation:LESTER, H., WON CHANG, H. Response of acetylcholine receptors to rapid photochemically produced increases in agonist concentration. Nature 266, 373–374 (1977). https://doi.org/10.1038/266373a0
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:105796
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:05 Oct 2020 19:49
Last Modified:05 Oct 2020 19:49

Repository Staff Only: item control page