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Role of voltage-sensitive receptors in nicotinic transmission

Lester, Henry A. and Koblin, Donald D. and Sheridan, Robert E. (1978) Role of voltage-sensitive receptors in nicotinic transmission. Biophysical Journal, 21 (3). pp. 181-194. ISSN 0006-3495. PMCID PMC1473691. https://resolver.caltech.edu/CaltechAUTHORS:20201005-113705979

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Abstract

This paper compares the conductance induced by bath-applied acetyl-choline (ACh) and by the same transmitter released from nerve terminals at Electrophorus electroplaques. For the former case, dose-response relations are characterized by the maximal agonist-induced conductance, rγ (130 mmho/cm²), and by the concentration which induces half this conductance; this concentration is termed K_(app) and equals 50 micron at -85 mV. For the latter case, neurally evoked postsynaptic currents (PSCs) are characterized by the peak conductance during strongly facilitated release, g_(PSC), and by the rate constant for decay, α. Since g_(PSC) roughly equals rγ, it is concluded that the PSC activates nearly all available receptor channels. These and other data agree with recent estimates that during the growth phase of the quantal response, (a) the ACh concentration is at least several hundred micromolar; and (b) most nearby channels are activated. However both α and K_(app) increase during depolarization, at a rate of about e-fold per 86 mV. These observations on voltage sensitivity suggest that a suprathreshold synaptic event is rapidly terminated because the action potential abruptly releases ACh molecules from receptors.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/s0006-3495(78)85518-0DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1473691/PubMed CentralArticle
ORCID:
AuthorORCID
Lester, Henry A.0000-0002-5470-5255
Additional Information:© 1978 The Biophysical Society. Published by Elsevier Under an Elsevier user license. Received for publication 23 June 1977 and in revised form 10 November 1977. We thank D. Williams for assistance with the animals and dissections, and P. Hartig, J. Heuser, M. Salpeter, and R. Zucker for helpful discussion. This work was supported by National Institutes of Health grant NS-11756; by a Grant-in-Aid from the Muscular Dystrophy Association, Inc.; by a Muscular Dystrophy Fellowship to D.D.K.; by an Alfred P. Sloan Fellowship and NIH Career Development Award NS272 to H.A.L.; and by a Spencer Foundation Fellowship to R.E.S.
Funders:
Funding AgencyGrant Number
NIHNS-11756
Muscular Dystrophy AssociationUNSPECIFIED
Alfred P. Sloan FoundationUNSPECIFIED
NIH Postdoctoral FellowshipNS-272
Spencer FoundationUNSPECIFIED
Issue or Number:3
PubMed Central ID:PMC1473691
Record Number:CaltechAUTHORS:20201005-113705979
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201005-113705979
Official Citation:H.A. Lester, D.D. Koblin, R.E. Sheridan, Role of voltage-sensitive receptors in nicotinic transmission, Biophysical Journal, Volume 21, Issue 3, 1978, Pages 181-194, ISSN 0006-3495, https://doi.org/10.1016/S0006-3495(78)85518-0.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:105797
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:05 Oct 2020 19:47
Last Modified:05 Oct 2020 19:47

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