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Two distinct mechanisms localise cyclin B transcripts in syncytial Drosophila embryos

Raff, Jordan W. and Whitfield, William G. F. and Glover, David M. (1990) Two distinct mechanisms localise cyclin B transcripts in syncytial Drosophila embryos. Development, 110 (4). pp. 1249-1261. ISSN 0950-1991. https://resolver.caltech.edu/CaltechAUTHORS:20201005-143626240

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Abstract

We demonstrate that two independent mechanisms act on maternally derived cyclin B transcripts to concentrate the transcripts at the posterior pole of the Drosophila oocyte and at the cortex of the syncytial embryo. The cortical accumulation occurs because the cyclin B transcript is concentrated around nuclei and comigrates with them to the cortex. The perinuclear localisation of the transcript is blocked by inhibitors of microtubule polymerisation and the transcript colocalises with microtubular structures during the cell cycle, suggesting that the transcript is associated either directly or indirectly with microtubules. Neither microtubules nor actin filaments are required to maintain the posterior concentration of cyclin B transcripts. Instead, this seems to depend on the association of the transcripts with a component of the posterior cytoplasm. The distribution pattern of the transcript at the posterior pole throughout embryogenesis and in a variety of mutant embryos suggests that this component is associated with polar granules.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://dev.biologists.org/content/110/4/1249PublisherArticle
ORCID:
AuthorORCID
Raff, Jordan W.0000-0002-4689-1297
Glover, David M.0000-0003-0956-0103
Additional Information:© 1990 by Company of Biologists. Accepted 7 September 1990. We thank Paul Lasko for generously providing the vasa antibody used in this study and for communicating results prior to publication. The mutant fly stocks were kindly donated by C. Nüsslein-Volhard and P. Lasko. We also thank Charles Girdham and Salud Llamazares for providing constructs.
Subject Keywords:cyclins, mRNA localisation, microtubules, polar granules, germ cells, Drosophila
Issue or Number:4
Record Number:CaltechAUTHORS:20201005-143626240
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201005-143626240
Official Citation:Two distinct mechanisms localise cyclin B transcripts in syncytial Drosophila embryos J.W. Raff, W.G. Whitfield, D.M. Glover Development 1990 110: 1249-1261
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:105810
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:05 Oct 2020 22:08
Last Modified:05 Oct 2020 22:08

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