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3' non-translated sequences in Drosophila cyclin B transcripts direct posterior pole accumulation late in oogenesis and peri-nuclear association in syncytial embryos

Dalby, Brian and Glover, David M. (1992) 3' non-translated sequences in Drosophila cyclin B transcripts direct posterior pole accumulation late in oogenesis and peri-nuclear association in syncytial embryos. Development, 115 (4). pp. 989-997. ISSN 0950-1991. https://resolver.caltech.edu/CaltechAUTHORS:20201005-143627215

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Abstract

We have characterised forms of the Drosophila cyclin B transcript that differ as a result of a splicing event which removes a nucleotide segment from the 3' untranslated region. In oogenesis, both cyclin A RNA and a shorter form of the cyclin B transcript are seen in the cells of the germarium that are undergoing mitosis. The shorter cyclin B transcript alone is then detectable in the presumptive oocyte until stages 7-8 of oogenesis. Both cyclin A RNA and a longer form of the cyclin B RNA are then synthesised in the nurse cells during stages 9-11, to be deposited in the oocyte during stages 11-12. These transcripts become evenly distributed throughout the oocyte cytoplasm but, in addition, those of cyclin B become concentrated at the posterior pole. Examination of the distributions of RNAs transcribed from chimeric cyclin genes indicates that sequences in the 3' untranslated region of the larger cyclin B RNA are required both for it to become concentrated at the posterior pole and to direct those transcripts in the body of the syncytial embryo to their peri-nuclear localisation. These sequences are disrupted by the splicing event which generates smaller cyclin B transcripts.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://dev.biologists.org/content/115/4/989.longPublisherArticle
ORCID:
AuthorORCID
Glover, David M.0000-0003-0956-0103
Additional Information:© 1992 by Company of Biologists. (Accepted 30 April 1992) We thank Tulle Hazelrigg for the gift of the adult testes cDNA library and Kevin O’Hare for the kind loan of the northern blot filters. We also thank Gabbie Maldonado-Codina for communicating unpublished data, Charles Girdham and Jordan Raff for comments and criticisms. This work was supported by the Cancer Research Campaign and a SERC studentship to B.D.
Funders:
Funding AgencyGrant Number
Cancer Research CampaignUNSPECIFIED
Science and Engineering Research Council (SERC)UNSPECIFIED
Subject Keywords:cyclin B, Drosophila, posterior pole, RNA localisation, oogenesis
Issue or Number:4
Record Number:CaltechAUTHORS:20201005-143627215
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201005-143627215
Official Citation:3′ non-translated sequences in Drosophila cyclin B transcripts direct posterior pole accumulation late in oogenesis and peri-nuclear association in syncytial embryos B. Dalby, D.M. Glover Development 1992 115: 989-997
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:105811
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:05 Oct 2020 22:21
Last Modified:05 Oct 2020 22:21

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