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Drosophila mutants in the 55 kDa regulatory subunit of protein phosphatase 2A show strongly reduced ability to dephosphorylate substrates of p34^(cdc2)

Mayer-Jaekel, Regina E. and Ohkura, Hiroyuki and Ferrigno, Paul and Andjelkovic, Natasa and Shiomi, Kensuke and Uemura, Tadashi and Glover, David M. and Hemmings, Brian A. (1994) Drosophila mutants in the 55 kDa regulatory subunit of protein phosphatase 2A show strongly reduced ability to dephosphorylate substrates of p34^(cdc2). Journal of Cell Science, 107 (9). pp. 2609-2616. ISSN 0021-9533. https://resolver.caltech.edu/CaltechAUTHORS:20201005-143628989

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Abstract

The 55 kDa regulatory subunit of Drosophila protein phosphatase 2A is located in the cytoplasm at all cell cycle stages, by the criterion of immunofluorescence. We are unable to detect significant change in protein phosphatase activity during the nuclear division cycle of syncytial embryos. However, cell cycle function of the enzyme is suggested by the mitotic defects exhibited by two Drosophila mutants, aar¹ and twins^P, defective in the gene encoding the 55 kDa subunit. The reduced levels of the 55 kDa subunit correlate with the loss of protein phosphatase 2A-like, okadaic acid-sensitive phosphatase activity of brain extracts against caldesmon and histone H1 phosphorylated by p34^(cdc2)/cyclin B kinase, but not against phosphorylase a. Thus the mitotic defects of aar¹ and twins^P are likely to result from the lack of dephosphorylation of specific substrates by protein phosphatase 2A.


Item Type:Article
Related URLs:
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https://jcs.biologists.org/content/107/9/2609.longPublisherArticle
ORCID:
AuthorORCID
Glover, David M.0000-0003-0956-0103
Alternate Title:Drosophila mutants in the 55 kDa regulatory subunit of protein phosphatase 2A show strongly reduced ability to dephosphorylate substrates of p34cdc2
Additional Information:© 1994 by Company of Biologists. (Received 14 March 1994 - Accepted 12 May 1994) We thank Bob MacKintosh and Patric Turowski for providing [³²P]phosphorylase a, Greg Moorhead for providing the p34^(cdc2)/cyclin B, and Stanislaw Zolnierowicz, Patric Turowski and Carl Smythe for commenting on the manuscript. R.M.-J. was supported for part of these studies by a short-term EMBO fellowship. H.O. and D.M.G. are grateful to the Cancer Research Campaign and the HSFP for support. P.F. was supported by grants from the MRC and HSFP (awarded to P. Cohen). K.S. was supported by a Fellowship of the Japan Society for the Promotion of Science for Junior Scientists; and T.U. is grateful to research grants from the Ministry of Education, Science and Culture of Japan.
Funders:
Funding AgencyGrant Number
European Molecular Biology Organization (EMBO)UNSPECIFIED
Cancer Research CampaignUNSPECIFIED
Human Frontier Science ProgramUNSPECIFIED
Medical Research Council (UK)UNSPECIFIED
Japan Society for the Promotion of Science (JSPS)UNSPECIFIED
Ministry of Education, Culture, Sports, Science and Technology (MEXT)UNSPECIFIED
Subject Keywords:mitosis, immunolocalization, cdc2
Issue or Number:9
Record Number:CaltechAUTHORS:20201005-143628989
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201005-143628989
Official Citation:Drosophila mutants in the 55 kDa regulatory subunit of protein phosphatase 2A show strongly reduced ability to dephosphorylate substrates of p34cdc2 R.E. Mayer-Jaekel, H. Ohkura, P. Ferrigno, N. Andjelkovic, K. Shiomi, T. Uemura, D.M. Glover, B.A. Hemmings Journal of Cell Science 1994 107: 2609-2616
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:105813
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:05 Oct 2020 22:31
Last Modified:05 Oct 2020 22:31

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