Glover, David M. and Zaha, Arnaldo and Stocker, Ann Jacob and Santelli, Roberto V. and Pueyo, Manuel T. and De Toledo, Sonia Maria and Lara, Francisco J. S. (1982) Gene amplification in Rhynchosciara salivary gland chromosomes. Proceedings of the National Academy of Sciences of the United States of America, 79 (9). pp. 2947-2951. ISSN 0027-8424. PMCID PMC346325. doi:10.1073/pnas.79.9.2947. https://resolver.caltech.edu/CaltechAUTHORS:20201005-143650238
![[img]](https://authors.library.caltech.edu/style/images/fileicons/application_pdf.png) |
PDF
- Published Version
See Usage Policy. 2MB |
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20201005-143650238
Abstract
Late in the fourth larval instar, several regions of the Rhynchosciara americana salivary gland chromosomes undergo "DNA puffing." We have constructed a library of cloned cDNAs synthesized from poly(A)⁺RNA isolated from salivary glands during the period of development when the DNA puffs are active. From this library we have studied clones representative of three genes active during this period but not active at earlier developmental periods of the gland. One of these genes is not amplified during the developmental process and encodes a 0.6-kilobase RNA molecule. The other two genes are located within the DNA-puff sites C3 and C8 and encode 1.25-kilobase and 1.95-kilobase RNA molecules, respectively. We estimate from the quantitation of transfer hybridization experiments that each of these genes undergoes 16-fold amplification during DNA puffing.
| Item Type: | Article | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Related URLs: |
| ||||||||||
| ORCID: |
| ||||||||||
| Additional Information: | © 1982 National Academy of Sciences. Communicated by Joseph G. Gall, February 1, 1982. We thank Mike Scott, Debbie Smith, and Mike Lovett for technical advice; Prof. W. Beermann for his continued support; and Claus Pelling, Cor Hollenberg, Werner Büsen, Orilio Leoncini, and Manuel Diaz for interesting discussions. This work was principally supported by a grant from the Stiftung Volkswagenwerk and also by grants from the Fundagio de Amparo a Pesquisa do Estado de Sao Paulo and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). D.M.G. held a fellowship under the exchange program of the Royal Society and the CNPq. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact. | ||||||||||
| Funders: |
| ||||||||||
| Subject Keywords: | cDNA clones/DNA puffs/C chromosome | ||||||||||
| Issue or Number: | 9 | ||||||||||
| PubMed Central ID: | PMC346325 | ||||||||||
| DOI: | 10.1073/pnas.79.9.2947 | ||||||||||
| Record Number: | CaltechAUTHORS:20201005-143650238 | ||||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20201005-143650238 | ||||||||||
| Official Citation: | Gene amplification in Rhynchosciara salivary gland chromosomes D M Glover, A Zaha, A J Stocker, R V Santelli, M T Pueyo, S M De Toledo, F J Lara Proceedings of the National Academy of Sciences May 1982, 79 (9) 2947-2951; DOI: 10.1073/pnas.79.9.2947 | ||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||
| ID Code: | 105816 | ||||||||||
| Collection: | CaltechAUTHORS | ||||||||||
| Deposited By: | George Porter | ||||||||||
| Deposited On: | 05 Oct 2020 22:03 | ||||||||||
| Last Modified: | 16 Nov 2021 18:46 |
Repository Staff Only: item control page
