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rough deal: a gene required for proper mitotic segregation in Drosophila

Karess, Roger E. and Glover, David M. (1989) rough deal: a gene required for proper mitotic segregation in Drosophila. Journal of Cell Biology, 109 (6). pp. 2951-2961. ISSN 0021-9525. PMCID PMC2115894. https://resolver.caltech.edu/CaltechAUTHORS:20201005-143650328

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Abstract

We describe a genetic locus rough deal (rod) in Drosophila melanogaster, identified by mutations that interfere with the faithful transmission of chromosomes to daughter cells during mitosis. Five mutant alleles were isolated, each associated with a similar set of mitotic abnormalities in the dividing neuroblasts of homozygous mutant larvae: high frequencies of aneuploid cells and abnormal anaphase figures, in which chromatids may lag, form bridges, or completely fail to separate. Surviving homozygous adults are sterile, and show cuticular defects associated with cell death, i.e., roughened eyes, sparse abdominal bristles, and notched wing margins. The morphological process of spermatogenesis is largely unaffected and motile sperm are produced, but meiocyte aneuploidy is common. The nature of the observed abnormalities in mitotic cells suggests that the reduced fidelity of chromosome transmission to the daughter cells is due to a failure in a mechanism involved in assuring the proper release of sister chromatids.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1083/jcb.109.6.2951DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2115894/PubMed CentralArticle
ORCID:
AuthorORCID
Glover, David M.0000-0003-0956-0103
Additional Information:© The Rockefeller University Press 1989. After the Initial Publication Period, RUP will grant to the public the non-exclusive right to copy, distribute, or display the Article under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode, or updates thereof. Received for publication 15 May 1989 and in revised form 14 August 1989. The authors gratefully acknowledge the excellent and diligent technical assistance of Alan Cheshire and Isabel Aguilera, as well as the stimulating mitotic discussions with M. Axton, A. Carpenter, P. D'Eustachio, C. Gonzalez, S. Hawley, H. Klein, and C. Sunkel. We thank T. Strecker, L. Goldstein, and A. Shearn for supplying fly stocks. This work was supported in part by a grant from the Medical Research Council of Great Britain. R. E. Karess was supported in the United States by grant GM37979 from the National Institutes of Health and a scholarship from the Pew Charitable Trust.
Funders:
Funding AgencyGrant Number
Medical Research Council (UK)UNSPECIFIED
NIHGM37979
Pew Charitable TrustUNSPECIFIED
Issue or Number:6
PubMed Central ID:PMC2115894
Record Number:CaltechAUTHORS:20201005-143650328
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201005-143650328
Official Citation:R E Karess, D M Glover; rough deal: a gene required for proper mitotic segregation in Drosophila.. J Cell Biol 1 December 1989; 109 (6): 2951–2961. doi: https://doi.org/10.1083/jcb.109.6.2951
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:105817
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:05 Oct 2020 22:00
Last Modified:05 Oct 2020 22:00

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