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polo encodes a protein kinase homolog required for mitosis in Drosophila

Llamazares, S. and Moreira, A. and Tavares, A. and Girdham, C. and Spruce, B. A. and Gonzalez, C. and Karess, R. E. and Glover, D. M. and Sunkel, C. E. (1991) polo encodes a protein kinase homolog required for mitosis in Drosophila. Genes and Development, 5 (12a). pp. 2153-2165. ISSN 0890-9369. doi:10.1101/gad.5.12a.2153.

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We show that mutation in polo leads to a variety of abnormal mitoses in Drosophila larval neuroblasts. These include otherwise normal looking mitotic spindles upon which chromosomes appear overcondensed; normal bipolar spindles with polyploid complements of chromosomes; bipolar spindles in which one pole can be unusually broad; and monopolar spindles. We have cloned the polo gene from a mutant allele carrying a P-element transposon and sequenced cDNAs corresponding to transcripts of the wild-type locus. The sequence shows that polo encodes a 577-amino-acid protein with an amino-terminal domain homologous to a serine-threonine protein kinase. polo transcripts are abundant in tissues and developmental stages in which there is extensive mitotic activity. The transcripts show no obvious spatial pattern of distribution in relation to the mitotic domains of cellularized embryos but are specifically concentrated in dividing cells in larval discs and brains. In the cell cycles of both syncytial and cellularized embryos, the polo kinase undergoes cell cycle-dependent changes in its distribution: It is predominantly cytoplasmic during interphase; it becomes associated with condensed chromosomes toward the end of prophase; and it remains associated with chromosomes until telophase, whereupon it becomes cytoplasmic.

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Glover, D. M.0000-0003-0956-0103
Additional Information:© 1991 Cold Spring Harbor Laboratory Press. The Authors acknowledge that six months after the full-issue publication date, the Article will be distributed under a Creative Commons CC-BY-NC License (Attribution-NonCommercial 4.0 International License, Received August 19, 1991; accepted September 12, 1991. We thank the Cancer Resarch Campaign of Great Britain and the Instituto de Investigacao Cientifica and the Junta Nacional de Investigaçâo Cientifica e Tecnologica (grant 67/676) of Portugal for supporting this work. We also thank Jordan Raft for carrying out some of the initial in situ hybridization experiments to localize polo transcripts in embryos and Ines Chavez for the screening of polo revertants. We thank Kevin O'Hare and Andrew Tomlinson for providing useful bits of P elements. The publication costs of this article were defrayed in part by payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact.
Funding AgencyGrant Number
Cancer Research CampaignUNSPECIFIED
Instituto de Investigacao CientificaUNSPECIFIED
Junta Nacional de Investigaçâo Cientifica e Tecnologica67/676
Subject Keywords:Mitosis; Drosophila; protein kinase; polo
Issue or Number:12a
Record Number:CaltechAUTHORS:20201005-143650434
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Official Citation:polo encodes a protein kinase homolog required for mitosis in Drosophila. S Llamazares, A Moreira, A Tavares, C Girdham, B A Spruce, C Gonzalez, R E Karess, D M Glover, and C E Sunkel Genes Dev. December 1991 5: 2153-2165; doi:10.1101/gad.5.12a.2153
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:105818
Deposited By: George Porter
Deposited On:05 Oct 2020 22:19
Last Modified:16 Nov 2021 18:46

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