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Failure of pronuclear migration and repeated divisions of polar body nuclei associated with MTOC defects in polo eggs of Drosophila

Riparbelli, M. G. and Callaini, G. and Glover, D. M. (2000) Failure of pronuclear migration and repeated divisions of polar body nuclei associated with MTOC defects in polo eggs of Drosophila. Journal of Cell Science, 113 (18). pp. 3341-3350. ISSN 0021-9533. https://resolver.caltech.edu/CaltechAUTHORS:20201005-153559731

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Abstract

The meiotic spindle of Drosophila oocytes is acentriolar but develops an unusual central microtubule organising centre (MTOC) at the end of meiosis I. In polo oocytes, this common central pole for the two tandem spindles of meiosis II was poorly organised and in contrast to wild-type failed to maintain its associated Pav-KLP motor protein. Furthermore, the polar body nuclei failed to arrest at metaphase, and the four products of female meiosis all underwent repeated haploid division cycles on anastral spindles. This was linked to a failure to form the astral array of microtubules with which the polar body chromosomes are normally associated. The MTOC associated with the male pronucleus was also defective in polo eggs, and the sperm aster did not grow. Migration of the female pronucleus did not take place and so a gonomeric spindle could not form. We discuss these findings in relation to the known roles of polo like kinases in regulating the behaviour of MTOCs.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://jcs.biologists.org/content/113/18/3341.longPublisherArticle
ORCID:
AuthorORCID
Callaini, G.0000-0003-2252-0309
Glover, D. M.0000-0003-0956-0103
Additional Information:© 2000 by Company of Biologists. Accepted 3 July 2000; published on WWW 22 August. This work was supported by grants from the Cancer Research Campaign, the BBSRC and the MURST. We thank Carmo Avides for her comments on the manuscript, and both Sharyn Endow and Endre Mathe for discussion about continued division cycles of the female meiotic products in various mutant backgrounds.
Funders:
Funding AgencyGrant Number
Cancer Research CampaignUNSPECIFIED
Biotechnology and Biological Sciences Research Council (BBSRC)UNSPECIFIED
Ministero dell'Università e della Ricerca Scientifico-Tecnologica (MURST)UNSPECIFIED
Subject Keywords:Drosophila, polo oocyte, Meiotic spindle, Sperm aster, Polar body
Issue or Number:18
Record Number:CaltechAUTHORS:20201005-153559731
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201005-153559731
Official Citation:Failure of pronuclear migration and repeated divisions of polar body nuclei associated with MTOC defects in polo eggs of Drosophila M.G. Riparbelli, G. Callaini, D.M. Glover Journal of Cell Science 2000 113: 3341-3350
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:105823
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:06 Oct 2020 14:25
Last Modified:06 Oct 2020 14:25

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