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Neurogenetic and genomic approaches reveal roles for Dpr/DIP cell adhesion molecules in Drosophila reproductive behavior

Brovero, Savannah G. and Fortier, Julia C. and Hu, Hongru and Lovejoy, Pamela C. and Newell, Nicole R. and Palmateer, Colleen M. and Tzeng, Ruei-Ying and Lee, Pei-Tseng and Zinn, Kai and Arbeitman, Michelle N. (2020) Neurogenetic and genomic approaches reveal roles for Dpr/DIP cell adhesion molecules in Drosophila reproductive behavior. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20201006-110721538

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Abstract

Drosophila reproductive behaviors are directed by fruitless neurons (fru P1 isoforms). A reanalysis of genomic studies shows that genes encoding dpr and DIP Immunoglobulin superfamily (IgSF) members are expressed in fru P1 neurons. Each fru P1and dpr/DIP (fru P1 ∩ dpr/DIP) overlapping expression pattern is similar in both sexes, with dimorphism in neuronal morphology and cell number. Behavioral studies of fru P1 ∩ dpr/DIP perturbation genotypes point to the mushroom body functioning together with the lateral protocerebral complex. Functionally, we find that perturbations of sex hierarchy genes and DIP-ε changes sex-specific morphology of fru P1 ∩ DIP-α neurons. A single-cell RNA-seq analysis shows that the DIPs have high expression in a restricted set of fru P1 neurons, whereas the dprs are expressed in larger set of neurons at intermediate levels, with a myriad of combinations.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/2020.10.02.323477DOIDiscussion Paper
ORCID:
AuthorORCID
Hu, Hongru0000-0003-0497-4796
Lovejoy, Pamela C.0000-0001-7315-5861
Palmateer, Colleen M.0000-0002-7254-0829
Tzeng, Ruei-Ying0000-0002-9009-9483
Zinn, Kai0000-0002-6706-5605
Arbeitman, Michelle N.0000-0002-2437-4352
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license. Posted October 04, 2020. The work presented was supported by NIH grants awarded to MNA: R01GM073039, R01GM116998, R03NS090184. This work was also supported by funds from the Biomedical Sciences Department, College of Medicine, Florida State University. We are grateful for the support. We appreciate that colleagues sent Drosophila stocks (Supplemental Table 7). Stocks were also obtained from the Bloomington Drosophila Stock Center (NIH P40OD018537). Several antibodies used in this study were obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University of Iowa, Department of Biology, Iowa City, IA 52242. We appreciate experimental assistance from Catherina Artikis.
Funders:
Funding AgencyGrant Number
NIHR01GM073039
NIHR01GM116998
NIHR03NS090184
Florida State UniversityUNSPECIFIED
NIHP40OD018537
Subject Keywords:Drosophila, courtship, reproductive behaviors, cell adhesion molecules (CAMs), single cell RNA-seq, IgSF
Record Number:CaltechAUTHORS:20201006-110721538
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201006-110721538
Official Citation:Neurogenetic and genomic approaches reveal roles for Dpr/DIP cell adhesion molecules in Drosophila reproductive behavior. Michelle Arbeitman, Savannah G Brovero, Julia C Fortier, Hongru Hu, Pamela C Lovejoy, Nicole R Newell, Colleen Palmateer, Ruei-Ying Tzeng, Pei-Tseng Lee, Kai Zinn. bioRxiv 2020.10.02.323477; doi: https://doi.org/10.1101/2020.10.02.323477
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:105838
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:07 Oct 2020 00:25
Last Modified:07 Oct 2020 00:25

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