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Metaphase Arrest with Centromere Separation in polo Mutants of Drosophila

Donaldson, Mary M. and Tavares, Álvaro A. M. and Ohkura, Hiroyuki and Deak, Peter and Glover, David M. (2001) Metaphase Arrest with Centromere Separation in polo Mutants of Drosophila. Journal of Cell Biology, 153 (4). pp. 663-676. ISSN 0021-9525. PMCID PMC2192380. doi:10.1083/jcb.153.4.663. https://resolver.caltech.edu/CaltechAUTHORS:20201007-123359292

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Abstract

The Drosophila gene polo encodes a conserved protein kinase known to be required to organize spindle poles and for cytokinesis. Here we report two strongly hypomorphic mutations of polo that arrest cells of the larval brain at a point in metaphase when the majority of sister kinetochores have separated by between 20–50% of the total spindle length in intact cells. In contrast, analysis of sister chromatid separation in squashed preparations of cells indicates that some 83% of sisters remain attached. This suggests the separation seen in intact cells requires the tension produced by a functional spindle. The point of arrest corresponds to the spindle integrity checkpoint; Bub1 protein and the 3F3/2 epitope are present on the separated kinetochores and the arrest is suppressed by a bub1 mutation. The mutant mitotic spindles are anastral and have assembled upon centrosomes that are associated with Centrosomin and the abnormal spindle protein (Asp), but neither with γ-tubulin nor CP190. We discuss roles for Polo kinase in recruiting centrosomal proteins and in regulating progression through the metaphase–anaphase checkpoint.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1083/jcb.153.4.663DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2192380/PubMed CentralArticle
ORCID:
AuthorORCID
Glover, David M.0000-0003-0956-0103
Additional Information:© 2001 The Rockefeller University Press. After the Initial Publication Period, RUP will grant to the public the non-exclusive right to copy, distribute, or display the Article under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode, or updates thereof. Submitted: 31 August 2000. Revised: 16 February 2001. Accepted: 19 March 2001. We thank Daryl Henderson for his comments on the manuscript. This work was supported by grants from the Cancer Research Campaign, the Biotechnology and Biological Sciences Research Council, and the European Union.
Funders:
Funding AgencyGrant Number
Cancer Research CampaignUNSPECIFIED
Biotechnology and Biological Sciences Research Council (BBSRC)UNSPECIFIED
European UnionUNSPECIFIED
Subject Keywords:mitosis • spindle checkpoint • Drosophila • Polo kinase • cell cycle
Issue or Number:4
PubMed Central ID:PMC2192380
DOI:10.1083/jcb.153.4.663
Record Number:CaltechAUTHORS:20201007-123359292
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201007-123359292
Official Citation:Mary M. Donaldson, Álvaro A.M. Tavares, Hiroyuki Ohkura, Peter Deak, David M. Glover; Metaphase Arrest with Centromere Separation in polo Mutants of Drosophila. J Cell Biol 14 May 2001; 153 (4): 663–676. doi: https://doi.org/10.1083/jcb.153.4.663
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:105885
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:07 Oct 2020 22:24
Last Modified:16 Nov 2021 18:47

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