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Drosophila Aurora B Kinase Is Required for Histone H3 Phosphorylation and Condensin Recruitment during Chromosome Condensation and to Organize the Central Spindle during Cytokinesis

Giet, Régis and Glover, David M. (2001) Drosophila Aurora B Kinase Is Required for Histone H3 Phosphorylation and Condensin Recruitment during Chromosome Condensation and to Organize the Central Spindle during Cytokinesis. Journal of Cell Biology, 152 (4). pp. 669-682. ISSN 0021-9525. PMCID PMC2195771. doi:10.1083/jcb.152.4.669. https://resolver.caltech.edu/CaltechAUTHORS:20201007-123359389

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Abstract

Aurora/Ipl1-related kinases are a conserved family of enzymes that have multiple functions during mitotic progression. Although it has been possible to use conventional genetic analysis to dissect the function of aurora, the founding family member in Drosophila (Glover, D.M., M.H. Leibowitz, D.A. McLean, and H. Parry. 1995. Cell. 81:95–105), the lack of mutations in a second aurora-like kinase gene, aurora B, precluded this approach. We now show that depleting Aurora B kinase using double-stranded RNA interference in cultured Drosophila cells results in polyploidy. aurora B encodes a passenger protein that associates first with condensing chromatin, concentrates at centromeres, and then relocates onto the central spindle at anaphase. Cells depleted of the Aurora B kinase show only partial chromosome condensation at mitosis. This is associated with a reduction in levels of the serine 10 phosphorylated form of histone H3 and a failure to recruit the Barren condensin protein onto chromosomes. These defects are associated with abnormal segregation resulting from lagging chromatids and extensive chromatin bridging at anaphase, similar to the phenotype of barren mutants (Bhat, M.A., A.V. Philp, D.M. Glover, and H.J. Bellen. 1996. Cell. 87:1103–1114.). The majority of treated cells also fail to undertake cytokinesis and show a reduced density of microtubules in the central region of the spindle. This is accompanied by a failure to correctly localize the Pavarotti kinesin-like protein, essential for this process. We discuss these conserved functions of Aurora B kinase in chromosome transmission and cytokinesis.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1083/jcb.152.4.669DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195771/PubMed CentralArticle
ORCID:
AuthorORCID
Giet, Régis0000-0001-9027-5849
Glover, David M.0000-0003-0956-0103
Additional Information:© 2001 The Rockefeller University Press. After the Initial Publication Period, RUP will grant to the public the non-exclusive right to copy, distribute, or display the Article under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode, or updates thereof. Submitted: 1 November 2000. Revised: 22 December 2000. Accepted: 29 December 2000. In Special Collection: JCB65: Cell Division, Cell Cycle, and Polarity We would like to thank Mark Jackman and Jonathan Pines for their great help in FACS® analysis with S2 cells and Hugo Bellen and Karen Schultze for continued interest and for kindly sending the antibarren antibody. The work was supported by a Marie-Curie Research Fellowship from the European Commission to R. Giet and by a program grant from the Cancer Research Campaign.
Funders:
Funding AgencyGrant Number
Marie Curie FellowshipUNSPECIFIED
Cancer Research CampaignUNSPECIFIED
Subject Keywords:Aurora B kinase • chromosome condensation • phospho-histone H3 • barren • cytokinesis
Issue or Number:4
PubMed Central ID:PMC2195771
DOI:10.1083/jcb.152.4.669
Record Number:CaltechAUTHORS:20201007-123359389
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201007-123359389
Official Citation:Régis Giet, David M. Glover; Drosophila Aurora B Kinase Is Required for Histone H3 Phosphorylation and Condensin Recruitment during Chromosome Condensation and to Organize the Central Spindle during Cytokinesis. J Cell Biol 19 February 2001; 152 (4): 669–682. doi: https://doi.org/10.1083/jcb.152.4.669
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:105886
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:07 Oct 2020 22:18
Last Modified:16 Nov 2021 18:47

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