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Drosophila Polo Kinase Is Required for Cytokinesis

Carmena, Mar and Riparbelli, Maria Giovanna and Minestrini, Gianluca and Tavares, Álvaro A. and Adams, Richard and Callaini, Giuliano and Glover, David M. (1998) Drosophila Polo Kinase Is Required for Cytokinesis. Journal of Cell Biology, 143 (3). pp. 659-671. ISSN 0021-9525. PMCID PMC2148135. https://resolver.caltech.edu/CaltechAUTHORS:20201007-123359671

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Abstract

A number of lines of evidence point to a predominance of cytokinesis defects in spermatogenesis in hypomorphic alleles of the Drosophila polo gene. In the pre-meiotic mitoses, cytokinesis defects result in cysts of primary spermatocytes with reduced numbers of cells that can contain multiple centrosomes. These are connected by a correspondingly reduced number of ring canals, structures formed by the stabilization of the cleavage furrow. The earliest defects during the meiotic divisions are a failure to form the correct mid-zone and mid-body structures at telophase. This is accompanied by a failure to correctly localize the Pavarotti kinesin- like protein that functions in cytokinesis, and of the septin Peanut and of actin to be incorporated into a contractile ring. In spite of these defects, cyclin B is degraded and the cells exit M phase. The resulting spermatids are frequently binuclear or tetranuclear, in which case they develop either two or four axonemes, respectively. A significant proportion of spermatids in which cytokinesis has failed may also show the segregation defects previously ascribed to polo1 mutants. We discuss these findings in respect to conserved functions for the Polo-like kinases in regulating progression through M phase, including the earliest events of cytokinesis.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1083/jcb.143.3.659DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2148135/PubMed CentralArticle
ORCID:
AuthorORCID
Carmena, Mar0000-0002-2352-1066
Callaini, Giuliano0000-0003-2252-0309
Glover, David M.0000-0003-0956-0103
Additional Information:© 1998 The Rockefeller University Press. After the Initial Publication Period, RUP will grant to the public the non-exclusive right to copy, distribute, or display the Article under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode, or updates thereof. Received for publication 12 March 1998 and in revised form 22 September 1998. We thank two unknown referees for their helpful criticism of the paper. Our work was supported by grants from the Cancer Research Campaign, BBSRC, and the European Union.
Funders:
Funding AgencyGrant Number
Cancer Research CampaignUNSPECIFIED
Biotechnology and Biological Sciences Research Council (BBSRC)UNSPECIFIED
European UnionUNSPECIFIED
Subject Keywords:Drosophila; Polo; cytokinesis; spermatogenesis; meiosis
Issue or Number:3
PubMed Central ID:PMC2148135
Record Number:CaltechAUTHORS:20201007-123359671
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201007-123359671
Official Citation:Mar Carmena, Maria Giovanna Riparbelli, Gianluca Minestrini, Álvaro M. Tavares, Richard Adams, Giuliano Callaini, David M. Glover; Drosophila Polo Kinase Is Required for Cytokinesis . J Cell Biol 2 November 1998; 143 (3): 659–671. doi: https://doi.org/10.1083/jcb.143.3.659
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:105889
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:08 Oct 2020 14:13
Last Modified:08 Oct 2020 14:13

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