Zheng, Liangbiao and Saunders, Robert D. C. and Fortini, Daniela and della Torre, Alessandra and Coluzzi, Mario and Glover, David M. and Kafatos, Fotis C. (1991) Low-resolution genome map of the malaria mosquito Anopheles gambiae. Proceedings of the National Academy of Sciences of the United States of America, 88 (24). pp. 11187-11191. ISSN 0027-8424. PMCID PMC53099. doi:10.1073/pnas.88.24.11187. https://resolver.caltech.edu/CaltechAUTHORS:20201007-123400111
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Abstract
We have microdissected divisions of the Anopheles gambiae polytene chromosomes, digested the DNAs with a restriction enzyme, and PCR-amplified the DNA fragments to generate a set of pooled probes, each corresponding to approximately 2% of the mosquito genome. These divisional probes were shown to have high complexity. Except for those derived from near the centromeres, they hybridize specifically with their chromosomal sites of origin. Thus, they can be used to map cloned DNAs by a dot blot procedure, which is much more convenient than in situ hybridization to polytene chromosomes. We discuss additional potential uses of these probes, such as easier isolation of molecular markers and genes, including those that cross-hybridize with clones available from other insects. It is expected that the probes will substantially accelerate molecular genetic analysis of this most important malaria vector.
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| Additional Information: | © 1991 National Academy of Sciences. Contributed by Fotis C. Kafatos, October 2, 1991. We are grateful to Drs. Douglas Seely, Louis Miller, Patricia Romans, and Frank Collins for generous gifts of half-gravid females and cloned DNA fragments and for useful suggestions. We thank Dr. Jeff Powell for helpful discussions, Bianca Klumpar and Marie Yuk-See for help with the figures, and Esther Fenejian and Zoe M. Kafatou for secretarial assistance. This work has been supported by grants from the John D. and Catherine T. MacArthur Foundation to F.C.K., from the European Community (Stimulation Action Programme) to F.C.K. and D.M.G., and from the Rockefeller Foundation to M.C. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact. | |||||||||
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| Issue or Number: | 24 | |||||||||
| PubMed Central ID: | PMC53099 | |||||||||
| DOI: | 10.1073/pnas.88.24.11187 | |||||||||
| Record Number: | CaltechAUTHORS:20201007-123400111 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20201007-123400111 | |||||||||
| Official Citation: | Low-resolution genome map of the malaria mosquito Anopheles gambiae L B Zheng, R D Saunders, D Fortini, A della Torre, M Coluzzi, D M Glover, F C Kafatos Proceedings of the National Academy of Sciences Dec 1991, 88 (24) 11187-11191; DOI: 10.1073/pnas.88.24.11187 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 105893 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | George Porter | |||||||||
| Deposited On: | 07 Oct 2020 19:53 | |||||||||
| Last Modified: | 16 Nov 2021 18:47 |
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