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Drosophila Klp67A is required for proper chromosome congression and segregation during meiosis I

Savoian, Matthew S. and Gatt, Melanie K. and Riparbelli, Maria G. and Callaini, Giuliano and Glover, David M. (2004) Drosophila Klp67A is required for proper chromosome congression and segregation during meiosis I. Journal of Cell Science, 117 (16). pp. 3669-3677. ISSN 0021-9533. doi:10.1242/jcs.01213. https://resolver.caltech.edu/CaltechAUTHORS:20201007-150756372

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[img] Video (AVI) (Movie 2. Time-lapse recording of a klp67A mutant during prometaphase) - Supplemental Material
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[img] Video (AVI) (Movie 3. Comparison of anaphase in a wild-type and klp67A mutant primary spermatocyte) - Supplemental Material
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[img] Video (AVI) (Movie 4. Example of merotelic malorientation in a living anaphase I klp67A mutant spermatocyte) - Supplemental Material
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Abstract

Drosophila Klp67A belongs to the Kip3 subfamily of Kinesin-type microtubule catastrophe factors. In primary spermatocytes, loss of klp67A leads to defects in karyokinesis and cytokinesis. We show that these cells formed disorganised, bipolar spindles that contained increased numbers of microtubules. The kinetochore fibres were wavy and bent, whereas astral microtubules appeared abnormally robust and formed cortical bundles. Time-lapse studies revealed that during biorientation, the chromosomes in klp67A mutant cells continued to reorient for about twice as long as those in control cells. Metaphase plates were poorly defined in the mutants and often formed at non-equatorial positions. Consistent with the above abnormalities in chromosome congression, we found that in wild-type cells Klp67A associated with prometaphase/metaphase kinetochores before redistributing to the central spindle at anaphase onset. Although the timing of this redistribution of kinetochores argues against a role in anaphase chromosome segregation, dyads in the mutants disjoined but exhibited greatly diminished poleward velocities. They travelled on average at approximately 34% of the velocity of their wild-type counterparts and often decondensed at non-polar locations. Hypomorphic mutations of klp67A may lead to segregation defects.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1242/jcs.01213DOIArticle
https://jcs.biologists.org/content/117/16/3669.supplementalPublisherSupporting Information
ORCID:
AuthorORCID
Savoian, Matthew S.0000-0002-2594-3879
Callaini, Giuliano0000-0003-2252-0309
Glover, David M.0000-0003-0956-0103
Additional Information:© The Company of Biologists Limited 2004. Accepted March 10, 2004. We would like to thank the laboratory of G. H. Karpen for the kind gift of the chicken anti-CID antibodies. We also thank E. Mathe for helpful discussions. This work was made possible by a MRC grant to D.M.G. and grants from PAR (University of Sienna) and MIUR to M.G.R. and G.C., respectively.
Funders:
Funding AgencyGrant Number
Medical Research Council (UK)UNSPECIFIED
University of SienaUNSPECIFIED
Ministero dell'Istruzione, dell'Università e della Ricerca (MIUR)UNSPECIFIED
Subject Keywords:Kinesin, Karyokinesis, Spindle, Kinetochore, Aster
Issue or Number:16
DOI:10.1242/jcs.01213
Record Number:CaltechAUTHORS:20201007-150756372
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201007-150756372
Official Citation:Drosophila Klp67A is required for proper chromosome congression and segregation during meiosis I Matthew S. Savoian, Melanie K. Gatt, Maria G. Riparbelli, Giuliano Callaini, David M. Glover Journal of Cell Science 2004 117: 3669-3677; doi: 10.1242/jcs.01213
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:105902
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:07 Oct 2020 22:36
Last Modified:16 Nov 2021 18:47

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