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Further evidence demonstrating that N-methyl-D-aspartate and kainate activate distinct ion channels

Fong, Tung Ming and Davidson, Norman and Lester, Henry A. (1989) Further evidence demonstrating that N-methyl-D-aspartate and kainate activate distinct ion channels. Synapse, 4 (1). pp. 88-95. ISSN 0887-4476. doi:10.1002/syn.890040110.

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Several excitatory amino acid receptors encoded by rat brain mRNA were expressed in Xenopus oocytes. Experimental protocols using an open channel blocker (MK‐801) were designed to test the common receptor‐channel hypothesis in which N‐methyl‐D‐aspartate (NMDA) and kainate activate the same ion channel but induce different open channel conformations with different ionic permeabilities. The present data demonstrate that NMDA exposes previously trapped MK‐801 molecules to the transmembrane field and accelerates their dissociation from the channel at positive potentials, while kainate lacks this effect, Therefore, kainate does not activate the same ion channel as NMDA does. Furthermore, differential inhibition of the NMDA response or the kainate response by the competitive antagonists D‐2‐amino‐5‐phosphonopentanoic acid (D‐AP5) and 6‐cyano‐7‐nitroquinoxaline‐2,3‐dione (CNQX) indicates that NMDA and kainate do not share the same binding site. Thus, these several lines of evidence demonstrate that two distinct receptor‐channels are activated by NMDA and kainate, respectively.

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Lester, Henry A.0000-0002-5470-5255
Additional Information:© 1989 Alan R. Liss, Inc. Manuscript accepted: 12 March 1989; Manuscript received: 11 January 1989.
Subject Keywords:Excitatory amino acid; Rat brain; Xenopus oocytes; Open channel blocker; Competitive antagonists
Issue or Number:1
Record Number:CaltechAUTHORS:20201012-122452479
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Official Citation:Fong, T.M., Davidson, N. and Lester, H.A. (1989), Further evidence demonstrating that N‐methyl‐D‐aspartate and kainate activate distinct ion channels. Synapse, 4: 88-95. doi:10.1002/syn.890040110
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:105980
Deposited By: Tony Diaz
Deposited On:12 Oct 2020 19:40
Last Modified:16 Nov 2021 18:48

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