A Caltech Library Service

Expression and modulation of voltage-gated calcium channels after RNA injection in Xenopus oocytes

Dascal, Nathan and Snutch, Terry P. and Lübbert, Hermann and Davidson, Norman and Lester, Henry A. (1986) Expression and modulation of voltage-gated calcium channels after RNA injection in Xenopus oocytes. Science, 231 (4742). pp. 1147-1150. ISSN 0036-8075. doi:10.1126/science.2418503.

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item:


Calcium ions flow into cells through several distinct classes of voltage-dependent calcium-selective channels. Such fluxes play important roles in electrical signaling at the cell membrane and in chemical signaling within cells. Further information about calcium channels was obtained by injecting RNA isolated from rat brain, heart and skeletal muscle into Xenopus oocytes. Macroscopic currents through voltage-operated calcium channels were resolved when the endogenous calcium-dependent chloride current was blocked by replacing external calcium with barium and chloride with methanesulfonate. The resulting barium current was insensitive to tetrodotoxin but was completely blocked by cadmium or cobalt. With both heart and brain RNA at least two distinct types of calcium ion conductance were found, distinguishable by their time course and inactivation properties. In oocytes injected with heart RNA, the slowly inactivating component was selectively blocked by the calcium-channel antagonist nifedipine. Barium ion currents induced by heart RNA were modulated by isoproterenol, cyclic adenosine monophosphate, and acetylcholine.

Item Type:Article
Related URLs:
URLURL TypeDescription
Lester, Henry A.0000-0002-5470-5255
Additional Information:© 1986 American Association for the Advancement of Science. 13 August 1985; accepted 17 December 1985. We thank J. P. Leonard, A. M. Gurney, J. M. Nerbonne, J. Nargeot, and D. Fambrough for helpful discussion. N.D. was a Myron R. Bantrell Fellow, T.P.S. was a postdoctoral fellow of the American Heart Association/Greater Los Angeles Affiliate, and H.L. was a fellow of the Deutsche Forschungsgemeinschaft. Supported by NIH grants GM-10991 and GM-29836.
Funding AgencyGrant Number
Natural Sciences and Engineering Research Council of Canada (NSERC)UNSPECIFIED
American Heart Association, Greater Los Angeles AffiliateUNSPECIFIED
Deutsche Forschungsgemeinschaft (DFG)UNSPECIFIED
Issue or Number:4742
Record Number:CaltechAUTHORS:20201012-152226204
Persistent URL:
Official Citation:Expression and modulation of voltage-gated calcium channels after RNA injection in Xenopus oocytes BY N DASCAL, TP SNUTCH, H LUBBERT, N DAVIDSON, HA LESTER Science 07 Mar 1986: Vol. 231, Issue 4742, pp. 1147-1150 DOI: 10.1126/science.2418503
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:105994
Deposited By: George Porter
Deposited On:13 Oct 2020 14:10
Last Modified:16 Nov 2021 18:48

Repository Staff Only: item control page