Dascal, Nathan and Snutch, Terry P. and Lübbert, Hermann and Davidson, Norman and Lester, Henry A. (1986) Expression and modulation of voltage-gated calcium channels after RNA injection in Xenopus oocytes. Science, 231 (4742). pp. 1147-1150. ISSN 0036-8075. doi:10.1126/science.2418503. https://resolver.caltech.edu/CaltechAUTHORS:20201012-152226204
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Abstract
Calcium ions flow into cells through several distinct classes of voltage-dependent calcium-selective channels. Such fluxes play important roles in electrical signaling at the cell membrane and in chemical signaling within cells. Further information about calcium channels was obtained by injecting RNA isolated from rat brain, heart and skeletal muscle into Xenopus oocytes. Macroscopic currents through voltage-operated calcium channels were resolved when the endogenous calcium-dependent chloride current was blocked by replacing external calcium with barium and chloride with methanesulfonate. The resulting barium current was insensitive to tetrodotoxin but was completely blocked by cadmium or cobalt. With both heart and brain RNA at least two distinct types of calcium ion conductance were found, distinguishable by their time course and inactivation properties. In oocytes injected with heart RNA, the slowly inactivating component was selectively blocked by the calcium-channel antagonist nifedipine. Barium ion currents induced by heart RNA were modulated by isoproterenol, cyclic adenosine monophosphate, and acetylcholine.
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| Additional Information: | © 1986 American Association for the Advancement of Science. 13 August 1985; accepted 17 December 1985. We thank J. P. Leonard, A. M. Gurney, J. M. Nerbonne, J. Nargeot, and D. Fambrough for helpful discussion. N.D. was a Myron R. Bantrell Fellow, T.P.S. was a postdoctoral fellow of the American Heart Association/Greater Los Angeles Affiliate, and H.L. was a fellow of the Deutsche Forschungsgemeinschaft. Supported by NIH grants GM-10991 and GM-29836. | ||||||||||||
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| Issue or Number: | 4742 | ||||||||||||
| DOI: | 10.1126/science.2418503 | ||||||||||||
| Record Number: | CaltechAUTHORS:20201012-152226204 | ||||||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20201012-152226204 | ||||||||||||
| Official Citation: | Expression and modulation of voltage-gated calcium channels after RNA injection in Xenopus oocytes BY N DASCAL, TP SNUTCH, H LUBBERT, N DAVIDSON, HA LESTER Science 07 Mar 1986: Vol. 231, Issue 4742, pp. 1147-1150 DOI: 10.1126/science.2418503 | ||||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||
| ID Code: | 105994 | ||||||||||||
| Collection: | CaltechAUTHORS | ||||||||||||
| Deposited By: | George Porter | ||||||||||||
| Deposited On: | 13 Oct 2020 14:10 | ||||||||||||
| Last Modified: | 16 Nov 2021 18:48 |
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