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cis-3,3'-Bis-[α-(trimethylammonium)methyl]azobenzene (cis-Bis-Q). Purification and properties at acetylcholine receptors of Electrophorus electroplaques

Nerbonne, Jeanne M. and Sheridan, Robert E. and Chabala, Lee D. and Lester, Henry A. (1983) cis-3,3'-Bis-[α-(trimethylammonium)methyl]azobenzene (cis-Bis-Q). Purification and properties at acetylcholine receptors of Electrophorus electroplaques. Molecular pharmacology, 23 (2). pp. 344-349. ISSN 0026-895X. https://resolver.caltech.edu/CaltechAUTHORS:20201012-160244665

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Abstract

The cis and trans isomers of the photoisomerizable compound, 3,3'-bis-[α-(trimethylammonium)methyl]azobenzene (Bis-Q), were purified by high-performance liquid chromatography using the ion-pair partitioning technique on a reverse-phase column. Solutions of cis-Bis-Q are stable at -20 degrees; at 25 degrees, thermal isomerization proceeds at a rate of 0.65%/day. cis-Bis-Q is less than 1% as potent a nicotinic agonist as the trans configuration. At concentrations of 1.5 microM or less, cis-Bis-Q exerts little or no blockade of the conductances induced by agonists. In voltage-clamped Electrophorus electroplaques exposed to cis-Bis-Q, laser flashes induce cis leads to trans photoisomerizations and increase the agonist-induced current by a factor of 20 within a few milliseconds.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://molpharm.aspetjournals.org/content/23/2/344.longPublisherArticle
ORCID:
AuthorORCID
Lester, Henry A.0000-0002-5470-5255
Additional Information:© 1983 American Society for Pharmacology and Experimental Therapeutics. We thank Roger Spencer for assistance in all phases of the project, Mauri Krouse for performing some of the electrophysiological measurements, and N. H. Wassermann and B. F. Erlanger for furnishing Bis-Q. This work was supported by National Institutes of Health Grant NS-11756. Recipient of a Fellowship from the American Heart Association. Recipient of a Fellowship from the Del E. Webb Foundation. Recipient of Research Career Development Award NS-272 from the National Institutes of Health.
Funders:
Funding AgencyGrant Number
NIHNS-11756
American Heart AssociationUNSPECIFIED
Del E. Webb FoundationUNSPECIFIED
NIH Postdoctoral FellowshipNS-272
Issue or Number:2
Record Number:CaltechAUTHORS:20201012-160244665
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201012-160244665
Official Citation:cis-3,3'-Bis-[alpha-(trimethylammonium)methyl]azobenzene (cis-Bis-Q). Purification and properties at acetylcholine receptors of Electrophorus electroplaques. J M Nerbonne, R E Sheridan, L D Chabala and H A Lester Molecular Pharmacology March 1, 1983, 23 (2) 344-349
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:105999
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:13 Oct 2020 14:37
Last Modified:13 Oct 2020 14:37

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