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Expression of functional sodium channels in stage II–III Xenopus oocytes

Krafte, Douglas S. and Lester, Henry A. (1989) Expression of functional sodium channels in stage II–III Xenopus oocytes. Journal of Neuroscience Methods, 26 (3). pp. 211-215. ISSN 0165-0270. doi:10.1016/0165-0270(89)90118-0.

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We have injected mRNA from rabbit brain into stage II–III Xenopus oocytes to determine whether they will translate exogenous RNA and incorporate functional ion channels into the membrane. Our results show that 48 h after RNA injection, functional voltage-dependent Na channels are present at sufficient densities to allow quantitative electrophysiological recording. The smaller oocytes have at least 2 experimental advantages over the stage V–VI oocytes normally used for electrophysiological experiments: (1) the smaller membrane capacitance (approximately 5-fold) allows a faster settling time following a voltage step and a more detailed kinetic analysis of membrane currents than was previously possible with the 2-microelectrode technique, and (2) roughly 8-fold less RNA is needed for each injection. Thus, the stage II–III Xenopus oocyte is a suitable preparation for the study of ion channels.

Item Type:Article
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Lester, Henry A.0000-0002-5470-5255
Additional Information:© 1989 Published by Elsevier. Received 20 April 1988, Revised 8 July 1988, Accepted 13 July 1988. We wish to thank Norman Davidson for helpful discussion. This work was supported by NS-11756, GM-10991, and an NRSA fellowship to D.S.K.
Funding AgencyGrant Number
National Research Service AwardUNSPECIFIED
Subject Keywords:Xenopus oocyte; Na channel; Inactivation; mRNA; Expression system
Issue or Number:3
Record Number:CaltechAUTHORS:20201012-160322367
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Official Citation:Douglas S. Krafte, Henry A. Lester, Expression of functional sodium channels in stage II–III Xenopus oocytes, Journal of Neuroscience Methods, Volume 26, Issue 3, 1989, Pages 211-215, ISSN 0165-0270,
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:106002
Deposited By: George Porter
Deposited On:13 Oct 2020 14:07
Last Modified:16 Nov 2021 18:49

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