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Overlapping and Distinct Roles of HAM Family Genes in Arabidopsis Shoot Meristems

Han, Han and Geng, Yuan and Guo, Lei and Yan, An and Meyerowitz, Elliot M. and Liu, Xing and Zhou, Yun (2020) Overlapping and Distinct Roles of HAM Family Genes in Arabidopsis Shoot Meristems. Frontiers in Plant Science, 11 . Art. No. 541968. ISSN 1664-462X. PMCID PMC7498855. https://resolver.caltech.edu/CaltechAUTHORS:20201012-163632477

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Abstract

In Arabidopsis shoot apical meristems (SAMs), a well-characterized regulatory loop between WUSCHEL (WUS) and CLAVATA3 (CLV3) maintains stem cell homeostasis by regulating the balance between cell proliferation and cell differentiation. WUS proteins, translated in deep cell layers, move into the overlaying stem cells to activate CLV3. The secreted peptide CLV3 then regulates WUS levels through a ligand-receptor mediated signaling cascade. CLV3 is specifically expressed in the stem cells and repressed in the deep cell layers despite presence of the WUS activator, forming an apical-basal polarity along the axis of the SAM. Previously, we proposed and validated a hypothesis that the HAIRY MERISTEM (HAM) family genes regulate this polarity, keeping the expression of CLV3 off in interior cells of the SAM. However, the specific role of each individual member of the HAM family in this process remains to be elucidated. Combining live imaging and molecular genetics, we have dissected the conserved and distinct functions of different HAM family members in control of CLV3 patterning in the SAMs and in the de novo shoot stem cell niches as well.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.3389/fpls.2020.541968DOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/pmc7498855/PubMed CentralArticle
ORCID:
AuthorORCID
Yan, An0000-0002-4676-5442
Meyerowitz, Elliot M.0000-0003-4798-5153
Liu, Xing0000-0002-0506-6821
Zhou, Yun0000-0003-4105-5174
Additional Information:© 2020 Han, Geng, Guo, Yan, Meyerowitz, Liu and Zhou. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Received: 11 March 2020; Accepted: 19 August 2020; Published: 04 September 2020. Edited by: Patrick Laufs, Institut National de la Recherche Agronomique (INRA), France. Reviewed by: David Jackson, Cold Spring Harbor Laboratory, United States. Mitsuhiro Aida, Kumamoto University, Japan. The authors thank the Purdue Bindley Bioscience Imaging Facility for access to the ZEISS LSM880 Laser scanning confocal microscope. This work is supported by Purdue University start-up and funds from Purdue Center for Plant Biology to YZ. The work in EM group was funded by the Howard Hughes Medical Institute. Author Contributions. HH, XL, AY, and YZ conceived the research direction. HH, YG, XL, and YZ performed the experiments. LG contributed research reagents. AY and EM discussed and commented on the experimental results. HH, XL, and YZ wrote the manuscript. AY and EM revised the manuscript. All authors contributed to the article and approved the submitted version. Data Availability Statement. All datasets generated for this study are included in the article/Supplementary Material. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Funders:
Funding AgencyGrant Number
Purdue Center for Plant BiologyUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Subject Keywords:shoot development, Arabidopsis, HAIRY MERISTEM, stem cells, confocal imaging, shoot apical meristems
PubMed Central ID:PMC7498855
Record Number:CaltechAUTHORS:20201012-163632477
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201012-163632477
Official Citation:Han, H., Geng, Y., Guo, L., Yan, A., Meyerowitz, E. M., Liu, X., & Zhou, Y. (2020). The Overlapping and Distinct Roles of HAM Family Genes in Arabidopsis Shoot Meristems. Frontiers in Plant Science, 11, 541968. https://doi.org/10.3389/fpls.2020.541968
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:106008
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:13 Oct 2020 16:54
Last Modified:13 Oct 2020 16:54

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