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Complementation of fission yeast cdc2^(ts) and cdc25^(ts) mutants identifies two cell cycle genes from Drosophila: a cdc2 homologue and string

Jimenez, Juan and Alphey, Luke and Nurse, Paul and Glover, David M. (1990) Complementation of fission yeast cdc2^(ts) and cdc25^(ts) mutants identifies two cell cycle genes from Drosophila: a cdc2 homologue and string. EMBO Journal, 9 (11). pp. 3565-3571. ISSN 0261-4189. PMCID PMC552107. doi:10.1002/j.1460-2075.1990.tb07567.x. https://resolver.caltech.edu/CaltechAUTHORS:20201013-133532081

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Abstract

We have exploited the universality of the molecular mechanisms that control entry into mitosis to clone the Drosophila melanogaster homologues of fission yeast Schizosaccharomyces pombe cell division control (cdc) genes by the complementation of temperature sensitive mutations. The Drosophila genes were expressed in S.pombe as cDNAs from the SP6 promoter. Successful recovery of complementing plasmids required that we first ‘adapt’ pooled plasmids from a Drosophila embryonic cDNA library for propagation in fission yeast by introducing an ars1‐LEU2 DNA fragment into the vector. This library was introduced into S.pombe cdc2 and cdc25 mutants, and plasmids isolated carrying cDNAs that complement these mutations. The gene that encodes the Drosophila cdc2 homologue maps to a single locus in the Drosophila genome at 31E on chromosome 2. It is expressed maternally to provide mRNA in syncytial embryos, and appears to be zygotically expressed in mitotically active regions of the cellularized embryo. We have isolated two different cDNAs that complement cdc25‐22. One corresponds to a transcript of string, previously described as the Drosophila homologue of cdc25, and the other to a gene that has not been previously characterized.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/j.1460-2075.1990.tb07567.xDOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC552107/PubMed CentralArticle
ORCID:
AuthorORCID
Glover, David M.0000-0003-0956-0103
Alternate Title:Complementation of fission yeast cdc2ts and cdc25ts mutants identifies two cell cycle genes from Drosophila: a cdc2 homologue and string
Additional Information:© 1990 European Molecular Biology Organization. Received on June 6, 1990; revised on August 8, 1990. We are very grateful to Nick Brown and Fotis Kafatos for providing the cDNA library, to Sergio Moreno for providing clones and strains, to Tamar Enoch and Stuart MacNeill for useful comments and discussions, and to Christian Lehner and Pat O'Farrell for communicating their results prior to publication. J.J. was funded by the EC (BAP-0441-UK contract). The OICRF and MRC provided support for P.N., and the CRC for D.M.G. and L.A.
Funders:
Funding AgencyGrant Number
European CommissionBAP-0441-UK
Medical Research Council (UK)UNSPECIFIED
Cancer Research CampaignUNSPECIFIED
Issue or Number:11
PubMed Central ID:PMC552107
DOI:10.1002/j.1460-2075.1990.tb07567.x
Record Number:CaltechAUTHORS:20201013-133532081
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201013-133532081
Official Citation:Jimenez, J., Alphey, L., Nurse, P. and Glover, D.M. (1990), Complementation of fission yeast cdc2ts and cdc25ts mutants identifies two cell cycle genes from Drosophila: a cdc2 homologue and string.. The EMBO Journal, 9: 3565-3571. doi:10.1002/j.1460-2075.1990.tb07567.x
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:106033
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:13 Oct 2020 21:47
Last Modified:16 Nov 2021 18:49

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