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The A- and B-type cyclins of Drosophila are accumulated and destroyed in temporally distinct events that define separable phases of the G2-M transition

Whitfield, William G. F. and Gonzalez, Cayetano and Maldonado-Codina, Gabriela and Glover, David M. (1990) The A- and B-type cyclins of Drosophila are accumulated and destroyed in temporally distinct events that define separable phases of the G2-M transition. EMBO Journal, 9 (8). pp. 2563-2572. ISSN 0261-4189. PMCID PMC552287. doi:10.1002/j.1460-2075.1990.tb07437.x. https://resolver.caltech.edu/CaltechAUTHORS:20201013-133532215

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Abstract

We show that the sequence of Drosophila cyclin B has greater identity with B‐type cyclins from other animal phyla than with Drosophila cyclin A, suggesting that the two cyclins have distinct roles that have been maintained in evolution. Cyclin A is not detectable in unfertilized eggs and is present at low levels prior to cellularization of the syncytial embryo. In contrast, the levels of cyclin B remain uniformly high throughout these developmental stages. In cells within cellularized embryos and the larval brain, cyclin A accumulates to peak levels in prophase and is degraded throughout the period in which chromosomes are becoming aligned on the metaphase plate. The degradation of cyclin B, on the other hand, does not occur until the metaphase‐anaphase transition. In cells arrested at c‐metaphase by treating with microtubule destabilizing drugs to prevent spindle formation, cyclin A has been degraded in the arrested cells, whereas cyclin B is maintained at high levels. These observations suggest that cyclin A has a role in the G2‐M transition that is independent of spindle formation, and that entry into anaphase is a key requirement for the degradation of cyclin B.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/j.1460-2075.1990.tb07437.xDOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC552287/PubMed CentralArticle
ORCID:
AuthorORCID
Glover, David M.0000-0003-0956-0103
Additional Information:© 1990 European Molecular Biology Organization. Received on March 12, 1990; revised on May 7, 1990. We would like to thank the Cancer Research Campaign for supporting this work. We are also grateful to Tim Hunt for his encouragement and for communicating unpublished work to us.
Funders:
Funding AgencyGrant Number
Cancer Research CampaignUNSPECIFIED
Subject Keywords:cell cycle/cyclin/Drosophila/mitosis
Issue or Number:8
PubMed Central ID:PMC552287
DOI:10.1002/j.1460-2075.1990.tb07437.x
Record Number:CaltechAUTHORS:20201013-133532215
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201013-133532215
Official Citation:Whitfield, W.G., Gonzalez, C., Maldonado‐Codina, G. and Glover, D.M. (1990), The A‐ and B‐type cyclins of Drosophila are accumulated and destroyed in temporally distinct events that define separable phases of the G2‐M transition.. The EMBO Journal, 9: 2563-2572. doi:10.1002/j.1460-2075.1990.tb07437.x
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:106034
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:13 Oct 2020 21:44
Last Modified:16 Nov 2021 18:49

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