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At least two mRNA species contribute to the properties of rat brain A-type potassium channel expressed in xenopus oocytes

Rudy, Bernardo and Hoger, Jeff H. and Lester, Henry A. and Davidson, Norman (1988) At least two mRNA species contribute to the properties of rat brain A-type potassium channel expressed in xenopus oocytes. Neuron, 1 (8). pp. 649-658. ISSN 0896-6273. doi:10.1016/0896-6273(88)90164-x. https://resolver.caltech.edu/CaltechAUTHORS:20201013-140643992

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Abstract

Fast transient K⁺ channels (A channels) of the type operating in the subthreshold region for Na⁺ action potential generation were expressed in Kenopus oocytes injected with rat brain poly(A) RNA. Sucrose gradient fractionation of the RNA separates mRNAs encoding A-currents (6–7 kb) from mRNAs encoding other voltage-dependent K⁺ channels. A-currents expressed with fractionated mRNA differ in kineticsAnd pharmacology from A-currents expressed with total mRNA. The original properties of the A-currents can be reconstituted when small mRNAs (2–4 kb) are added to the large mRNA fraction. Thus the properties of the A-currents expressed with total poly(A) RNA depend on the presence of more than one mRNA species. mRNA(s) present in the large RNA fraction must encode channel subunits since they express an A-current by themselves. The small mRNA(s) may encode a second subunit(s) or a factor, such as an enzymatic activity that modulates the properties of the channels, which could play a role in generating A-channel functional diversity.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/0896-6273(88)90164-xDOIArticle
ORCID:
AuthorORCID
Lester, Henry A.0000-0002-5470-5255
Additional Information:© 1988 Cell Press. Received 6 February 1988, Revised 30 July 1988. We wish to thank Dr. R. Llinas for his support during this project; D. Krafte, T. Snutch, A. Coldin, J. Leonard, and R. J. Leonard for sharing their expertise; and J. Campanelli and A. Kamb for their interest and insightful comments and suggestions. This research was supported by USPHS grant GM26976 To B. R., GM10991 to N. D., and GM29836 to H. A. L. J. H. H. is a fellow of the American Cancer Society.
Funders:
Funding AgencyGrant Number
NIHGM-26976
NIHGM-10991
NIHGM-29836
American Cancer SocietyUNSPECIFIED
Issue or Number:8
DOI:10.1016/0896-6273(88)90164-x
Record Number:CaltechAUTHORS:20201013-140643992
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201013-140643992
Official Citation:Bernardo Rudy, Jeff H. Hoger, Henry A. Lester, Norman Davidson, At least two mRNA species contribute to the properties of rat brain A-type potassium channel expressed in xenopus oocytes, Neuron, Volume 1, Issue 8, 1988, Pages 649-658, ISSN 0896-6273, https://doi.org/10.1016/0896-6273(88)90164-X.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:106035
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:13 Oct 2020 22:10
Last Modified:16 Nov 2021 18:49

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