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A rat brain Na⁺ channel α subunit with novel gating properties

Auld, Vanessa J. and Goldin, Alan L. and Krafte, Douglas S. and Marshall, John and Dunn, James M. and Catterall, William A. and Lester, Henry A. and Davidson, Norman and Dunn, Robert J. (1988) A rat brain Na⁺ channel α subunit with novel gating properties. Neuron, 1 (6). pp. 449-461. ISSN 0896-6273. doi:10.1016/0896-6273(88)90176-6.

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We have constructed a full-length rat brain Na+ channel α subunit cDNA that differs from the previously reported a subunit of Noda et al. at 6 amino acid positions. Transcription of the cDNA in vitro and injection into Xenopus oocytes resulted in the synthesis of functional Na⁺ channels. Although the single-channel conductance of the channels resulting from cloned cDNA was the same as that of channels resulting from injection of rat brain RNA, we observed two significant differences in the gating properties of the channels. The Na+ currents from cloned cDNA displayed much slower macroscopic inactivation compared with those from rat brain mRNA. In addition, the current-voltage relationship for currents from cloned cDNA was shifted 20–25 mV in the depolarizing direction compared with currents from rat brain RNA. Coinjection of low MW rat brain RNA restored normal inactivation of the channels indicating the presence of a component, either a structural subunit of the channel complex or a modifying enzyme, necessary for normal gating of the channel.

Item Type:Article
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Lester, Henry A.0000-0002-5470-5255
Additional Information:© 1988 Cell Press. Received 14 April 1988, Revised 25 May 1988. We thank David Anderson for providing a rat brain cDNA library, Steve Clark for help with computer analysis, Shane Climie for sequencing advice, Jim Ingles for pEMBL-18, Derek van der Kooy for providing rat tissue, and Gayle Bodourian for manuscript preparation. This work was supported by the Medical Research Council of Canada (R. J. D.), National Institutes of Health Grants NS111756, and GM10991 (H. A. L. and N. D.), and the Muscular Dystrophy Association (H. A. L. and N. D.). A. L. G. is a Lucille P Markey Scholar, and this work was supported In part by a grant from the Lucille P. Markey Charitable Trust. D. S. K. is a Fellow of the National Institutes of Health.
Funding AgencyGrant Number
Medical Research Council of CanadaUNSPECIFIED
Muscular Dystrophy AssociationUNSPECIFIED
Lucille P. Markey Charitable TrustUNSPECIFIED
NIH Postdoctoral FellowshipUNSPECIFIED
Issue or Number:6
Record Number:CaltechAUTHORS:20201013-140644253
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Official Citation:Vanessa J. Auld, Alan L. Goldin, Douglas S. Krafte, John Marshall, James M. Dunn, William A. Catterall, Henry A. Lester, Norman Davidson, Robert J. Dunn, A rat brain na+ channel α subunit with novel gating properties, Neuron, Volume 1, Issue 6, 1988, Pages 449-461, ISSN 0896-6273,
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:106036
Deposited By: George Porter
Deposited On:13 Oct 2020 22:08
Last Modified:16 Nov 2021 18:49

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