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A neuropeptide precursor in cerebellum: proenkephalin exists in subpopulations of both neurons and astrocytes

Spruce, Barbara A. and Curtis, Rory and Wilkin, Graham P. and Glover, David M. (1990) A neuropeptide precursor in cerebellum: proenkephalin exists in subpopulations of both neurons and astrocytes. EMBO Journal, 9 (6). pp. 1787-1795. ISSN 0261-4189. PMCID PMC551883. doi:10.1002/j.1460-2075.1990.tb08303.x. https://resolver.caltech.edu/CaltechAUTHORS:20201013-145241437

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Abstract

The adult rat cerebellum has minimal enkephalin immunoreactivity and is devoid of opiate‐binding activity. Using novel monoclonal antibodies to the mammalian enkephalin precursor, we describe the immunofluorescent detection of proenkephalin, in the absence of mature enkephalin peptides, in subpopulations of rat cerebellar neurons and astrocytes. In cryostat sections, neurons that express proenkephalin include Golgi cells, macroneurons within deep cerebellar nuclei and a subpopulation of Purkinje cells. Proenkephalin messenger RNA and protein are present in subpopulations of both grey and white matter astrocytes, but not Bergmann glia. In dissociated glial culture, proenkephalin is expressed in process‐bearing astrocytes, apparently in association with a subset of intermediate filaments. Proenkephalin within astrocytes is not seen until the second postnatal week and increases through to adulthood. Neuropeptide gene expression adds to the growing range of neuronal‐type properties glial cells can display.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/j.1460-2075.1990.tb08303.xDOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC551883/PubMed CentralArticle
ORCID:
AuthorORCID
Glover, David M.0000-0003-0956-0103
Additional Information:© 1990 European Molecular Biology Organization. Received on November 10, 1989; revised on March 20, 1990. We thank Professor Martin Raff for helpful discussion. We are grateful to Dr M. Comb and the late Dr E. Herbert for the full-length human proenkephalin cDNA clone, without which this latest series of antibodies would not have been possible. We thank Dr U. Guebler (Hoffman la Roche Inc.) for providing us with bovine proenkephalin cDNA and we particularly thank Dr S. Sabol for rat proenkephalin cDNA together with sense and antisense transcription vectors which enabled speedy achievement of our in situ data. B.A.S. and D.M.G. acknowledge the Medical Research Council for support through a Project Grant. D.M.G. thanks the Cancer Research Campaign for provision of the confocal microscope. R.C. is in receipt of a Science and Engineering Research Council Studentship. G.P.W. acknowledges the support of the Medical Research Council, the Multiple Sclerosis Society, and the International Spinal Research Trust.
Funders:
Funding AgencyGrant Number
Medical Research Council (UK)UNSPECIFIED
Cancer Research CampaignUNSPECIFIED
Science and Engineering Research Council (SERC)UNSPECIFIED
Multiple Sclerosis SocietyUNSPECIFIED
International Spinal Research TrustUNSPECIFIED
Subject Keywords:glia; in situ hybridization; intermediate filaments; monoclonal antibodies; opioid gene
Issue or Number:6
PubMed Central ID:PMC551883
DOI:10.1002/j.1460-2075.1990.tb08303.x
Record Number:CaltechAUTHORS:20201013-145241437
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201013-145241437
Official Citation:Spruce, B., Curtis, R., Wilkin, G. and Glover, D. (1990), A neuropeptide precursor in cerebellum: proenkephalin exists in subpopulations of both neurons and astrocytes.. The EMBO Journal, 9: 1787-1795. doi:10.1002/j.1460-2075.1990.tb08303.x
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:106042
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:13 Oct 2020 22:42
Last Modified:16 Nov 2021 18:49

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