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Cis-acting sequences sufficient for correct tissue and temporal specificity of larval serum protein 1 genes of Drosophila

Delaney, Stephen J. and Sunkel, Claudio E. and Genova-Seminova, Ginka K. and Davies, Jane E. and Glover, David M. (1987) Cis-acting sequences sufficient for correct tissue and temporal specificity of larval serum protein 1 genes of Drosophila. EMBO Journal, 6 (12). pp. 3849-3854. ISSN 0261-4189. PMCID PMC553858. https://resolver.caltech.edu/CaltechAUTHORS:20201013-145241670

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Abstract

We have constructed hybrid genes in which the coding region of the bacterial gene chloramphenicol acetyl transferase (CAT) has been linked to varying lengths of upstream sequences of Drosophila genes for larval serum sequence 1 (LSP1). These have been inserted into a P‐element transformation vector and subsequently transferred into the germ‐line of recipient flies. Transformants carrying the CAT gene linked to 1650 bp, 570 bp or 377 bp of upstream LSP1α sequences, or 745 bp or 471 bp of upstream β sequences express CAT with the same developmental and tissue specificity as the endogenous LSP1 genes. Constructs having only 66 bp of upstream LSP1β sequences, however, show extremely low levels of CAT expression in tissues and at developmental stages in which LSP1 is not expressed. We discuss the significance of short regions of homology between the DNA upstream of the α and β genes, which lie within the regions identified by the transformation experiments as being required for the cis‐regulation of LSP1 synthesis.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/j.1460-2075.1987.tb02722.xDOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC553858/PubMed CentralArticle
ORCID:
AuthorORCID
Glover, David M.0000-0003-0956-0103
Additional Information:© 1987 European Molecular Biology Organization. Received on August 18, 1987; revised on September 15, 1987. We wish to thank Trevor Jowett, David Roberts and Debbie Smith for their interest and discussions. We also thank the Cancer Research Campaign for supporting this work and for a Career Development Award to D.M.G. G.G.-S. was a visitor under the Anglo-Bulgarian Cultural Exchange Scheme of the Bulgarian Ministry of Education and the British Council.
Funders:
Funding AgencyGrant Number
Cancer Research CampaignUNSPECIFIED
Anglo-Bulgarian Cultural Exchange SchemeUNSPECIFIED
Subject Keywords:Drosophila/LSPI genes/transformation/CAT/developmental specificity
Issue or Number:12
PubMed Central ID:PMC553858
Record Number:CaltechAUTHORS:20201013-145241670
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201013-145241670
Official Citation:Delaney, S.J., Sunkel, C.E., Genova‐Seminova, G.K., Davies, J.E. and Glover, D.M. (1987), Cis‐acting sequences sufficient for correct tissue and temporal specificity of larval serum protein 1 genes of Drosophila. The EMBO Journal, 6: 3849-3854. doi:10.1002/j.1460-2075.1987.tb02722.x
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:106044
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:13 Oct 2020 22:23
Last Modified:13 Oct 2020 22:23

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