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Widely differing degrees of sequence conservation of the two types of rDNA insertion within the melanogaster species sub-group of Drosophila

Roiha, Heli and Read, Christopher A. and Browne, Michael J. and Glover, David M. (1983) Widely differing degrees of sequence conservation of the two types of rDNA insertion within the melanogaster species sub-group of Drosophila. EMBO Journal, 2 (5). pp. 721-726. ISSN 0261-4189. PMCID PMC555176. https://resolver.caltech.edu/CaltechAUTHORS:20201013-155619512

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Abstract

We have examined the distribution of sequences homologous to the type I and type II rDNA insertions of Drosophila melanogaster in its sibling species. Each of the six species we have examined has sequences homologous to the type I insertion, which have undergone extensive divergence by the criterion of their EcoRI, BstI and HindIII restriction patterns. We have isolated cosmid clones containing type I sequences from D. simulans and D. mauritiana, the two species most closely related to D. melanogaster. Southern hybridisation analysis of these clones indicates that, as in D. melanogaster, the type I sequences can exist independently of rDNA and can also dissociate to give sub‐components homologous to the right hand segment of the D. melanogaster type I insertion. The type II sequences, on the other hand are present in five out of the six species, but their restriction endonuclease cleavage profile is highly conserved. The differences in the degree of conservation of the two types of insertion sequence are discussed.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/j.1460-2075.1983.tb01491.xDOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC555176/PubMed CentralArticle
ORCID:
AuthorORCID
Glover, David M.0000-0003-0956-0103
Additional Information:© 1983 European Molecular Biology Organization. Received on 18 February 1983. This work was supported by grants from the Medical Research Council and the Cancer Research Campaign (CRC). DMG holds a Career Development Award from the CRC. MJB was a visiting worker from Beecham Pharmaceuticals.
Funders:
Funding AgencyGrant Number
Medical Research Council (UK)UNSPECIFIED
Cancer Research CampaignUNSPECIFIED
Subject Keywords:rDNA insertions/Drosophila melanogaster/sibling species
Issue or Number:5
PubMed Central ID:PMC555176
Record Number:CaltechAUTHORS:20201013-155619512
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201013-155619512
Official Citation:Roiha, H., Read, C.A., Browne, M.J. and Glover, D.M. (1983), Widely differing degrees of sequence conservation of the two types of rDNA insertion within the melanogaster species sub‐group of Drosophila. The EMBO Journal, 2: 721-726. doi:10.1002/j.1460-2075.1983.tb01491.x
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:106048
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:14 Oct 2020 14:22
Last Modified:14 Oct 2020 14:22

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