Binding, Horst and Weber, Hans Jürgen (1974) The isolation, regeneration and fusion of Phycomyces protoplasts. Molecular and General Genetics MGG, 135 (3). pp. 273-276. ISSN 0026-8925. doi:10.1007/bf00268622. https://resolver.caltech.edu/CaltechAUTHORS:20201020-080005564
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Abstract
Germlings of Phycomyces blakesleeanus were converted to protoplasts using an enzyme solution of chitinase and chitosanase. The protoplasts were regenerated to normal growing mycelia. Protoplasts of different auxotrophic strains fused in diluted natural seawater and in Ca(NO₃)₂ solutions. 36 prototrophic strains were isolated (Table 1). Their heterokaryotic nature was proved by the segregation of the markers in vegetative spores.
| Item Type: | Article | ||||||
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| Additional Information: | © 1974 Springer Science and Business Media LLC. Received 27 December 1974; Published 01 September 1975; Issue Date September 1974. | ||||||
| Subject Keywords: | Enzyme; Chitinase; Enzyme Solution; Natural Seawater; Phycomyces | ||||||
| Issue or Number: | 3 | ||||||
| DOI: | 10.1007/bf00268622 | ||||||
| Record Number: | CaltechAUTHORS:20201020-080005564 | ||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20201020-080005564 | ||||||
| Official Citation: | Binding, H., Weber, H.J. The isolation, regeneration and fusion of Phycomyces protoplasts. Molec. Gen. Genet. 135, 273–276 (1974). https://doi.org/10.1007/BF00268622 | ||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||
| ID Code: | 106163 | ||||||
| Collection: | CaltechAUTHORS | ||||||
| Deposited By: | Tony Diaz | ||||||
| Deposited On: | 20 Oct 2020 19:07 | ||||||
| Last Modified: | 16 Nov 2021 18:51 |
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