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The isolation, regeneration and fusion of Phycomyces protoplasts

Binding, Horst and Weber, Hans Jürgen (1974) The isolation, regeneration and fusion of Phycomyces protoplasts. Molecular and General Genetics MGG, 135 (3). pp. 273-276. ISSN 0026-8925. doi:10.1007/bf00268622.

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Germlings of Phycomyces blakesleeanus were converted to protoplasts using an enzyme solution of chitinase and chitosanase. The protoplasts were regenerated to normal growing mycelia. Protoplasts of different auxotrophic strains fused in diluted natural seawater and in Ca(NO₃)₂ solutions. 36 prototrophic strains were isolated (Table 1). Their heterokaryotic nature was proved by the segregation of the markers in vegetative spores.

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Additional Information:© 1974 Springer Science and Business Media LLC. Received 27 December 1974; Published 01 September 1975; Issue Date September 1974.
Subject Keywords:Enzyme; Chitinase; Enzyme Solution; Natural Seawater; Phycomyces
Issue or Number:3
Record Number:CaltechAUTHORS:20201020-080005564
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Official Citation:Binding, H., Weber, H.J. The isolation, regeneration and fusion of Phycomyces protoplasts. Molec. Gen. Genet. 135, 273–276 (1974).
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:106163
Deposited By: Tony Diaz
Deposited On:20 Oct 2020 19:07
Last Modified:16 Nov 2021 18:51

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