CaltechAUTHORS
  A Caltech Library Service

The isolation, regeneration and fusion of Phycomyces protoplasts

Binding, Horst and Weber, Hans Jürgen (1974) The isolation, regeneration and fusion of Phycomyces protoplasts. Molecular and General Genetics MGG, 135 (3). pp. 273-276. ISSN 0026-8925. https://resolver.caltech.edu/CaltechAUTHORS:20201020-080005564

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20201020-080005564

Abstract

Germlings of Phycomyces blakesleeanus were converted to protoplasts using an enzyme solution of chitinase and chitosanase. The protoplasts were regenerated to normal growing mycelia. Protoplasts of different auxotrophic strains fused in diluted natural seawater and in Ca(NO₃)₂ solutions. 36 prototrophic strains were isolated (Table 1). Their heterokaryotic nature was proved by the segregation of the markers in vegetative spores.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1007/bf00268622DOIArticle
Additional Information:© 1974 Springer Science and Business Media LLC. Received 27 December 1974; Published 01 September 1975; Issue Date September 1974.
Subject Keywords:Enzyme; Chitinase; Enzyme Solution; Natural Seawater; Phycomyces
Issue or Number:3
Record Number:CaltechAUTHORS:20201020-080005564
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201020-080005564
Official Citation:Binding, H., Weber, H.J. The isolation, regeneration and fusion of Phycomyces protoplasts. Molec. Gen. Genet. 135, 273–276 (1974). https://doi.org/10.1007/BF00268622
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:106163
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:20 Oct 2020 19:07
Last Modified:20 Oct 2020 19:07

Repository Staff Only: item control page