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Cooperation-based sperm clusters mediate sperm oviduct entry and fertilization

Qu, Yongcun and Chen, Qi and Guo, Shanshan and Ma, Chiyuan and Lu, Yonggang and Shi, Junchao and Liu, Shichao and Zhou, Tong and Noda, Taichi and Qian, Jingjing and Zhang, Liwen and Zhu, Xili and Lei, Xiaohua and Cao, Yujing and Li, Wei and Li, Wei and Plachta, Nicolas and Matzuk, Martin M. and Ikawa, Masahito and Duan, Enkui and Zhang, Ying and Wang, Hongmei (2020) Cooperation-based sperm clusters mediate sperm oviduct entry and fertilization. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20201021-072733806

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Abstract

Sperm cooperation has been observed in multiple species, yet its existence and benefit for reproductive success in mammals remains underexplored. Here, combining tissue-clearing with deep three-dimensional imaging, we demonstrate that postcopulatory mouse sperm congregate into unidirectional sperm cooperative clusters at the utero-tubal junction (UTJ), a key physical barrier for passage into the oviduct. Reducing sperm number in male mice by unilateral vasoligation or busulfan-treatment impairs sperm cluster formation and oviduct entry. Interestingly, sperm derived from Tex101-/- mouse has normal number, motility and morphology, yet they cannot form sperm cluster and fail to pass through the UTJ, which is at least in part due to the altered tail beating pattern of the Tex101-/- sperm. Moreover, Tex101-/- sperm's defect in oviduct entry cannot be rescued by the presence of wild-type (WT) sperm in the same uteri by sequential mating, suggesting sperm cooperative cluster as an essential behavior contributing to male fertility, which could be related to human infertility or subfertility.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/2020.10.18.344275DOIDiscussion Paper
ORCID:
AuthorORCID
Chen, Qi0000-0001-6353-9589
Li, Wei0000-0002-6235-0749
Li, Wei0000-0001-7864-404X
Plachta, Nicolas0000-0002-5035-7425
Ikawa, Masahito0000-0001-9859-6217
Zhang, Ying0000-0003-4082-5026
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. Posted October 19, 2020. This work was supported by the National Key Research and Development Program of China (2019YFA0802600 to YZ, 2018YFC1004500 to YZ, 2016YFA0500903 to ED, 2017YFC1001401 to ED, 2015CB943003 to YZ), the Strategic Priority Research Program of the CAS (XDA16020700 to HW) National Basic Research Program of China (81490742 to ED), National Natural Science Foundation of China (81490741 to HW, 31671201 to YZ, 31671568 to ED), Youth Innovation Promotion Association, CAS (Grant No. 2016081 to YZ), NIH (R01HD092431 to QC). We thank Shiwen Li for the help of image processing. The authors have declared no competing interest.
Funders:
Funding AgencyGrant Number
National Key Research and Development Program of China2019YFA0802600
National Key Research and Development Program of China2018YFC1004500
National Key Research and Development Program of China2016YFA0500903
National Key Research and Development Program of China2017YFC1001401
National Key Research and Development Program of China2015CB943003
Chinese Academy of SciencesXDA16020700
National Basic Research Program of China81490742
National Natural Science Foundation of China81490741
National Natural Science Foundation of China31671201
National Natural Science Foundation of China31671568
Chinese Academy of Sciences2016081
NIHR01HD092431
Record Number:CaltechAUTHORS:20201021-072733806
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201021-072733806
Official Citation:Cooperation-based sperm clusters mediate sperm oviduct entry and fertilization. Yongcun Qu, Qi Chen, Shanshan Guo, Chiyuan Ma, Yonggang Lu, Junchao Shi, Shichao Liu, Tong Zhou, Taichi Noda, Jingjing Qian, Liwen Zhang, Xili Zhu, Xiaohua Lei, Yujing Cao, Wei Li, Wei Li, Nicolas Plachta, Martin Matthew Matzuk, Masahito Ikawa, Enkui Duan, Ying Zhang, Hongmei Wang. bioRxiv 2020.10.18.344275; doi: https://doi.org/10.1101/2020.10.18.344275
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:106176
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:21 Oct 2020 16:55
Last Modified:21 Oct 2020 16:55

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