Chung, Bong Hyun and Arnold, Frances H. (1992) Metal Affinity Partitioning of Phosphoproteins in Aqueous Two-Phase Systems. In: Biochemical Engineering for 2001. Springer Japan , Tokyo, pp. 557-559. ISBN 9784431681823. https://resolver.caltech.edu/CaltechAUTHORS:20201023-182805331
Full text is not posted in this repository. Consult Related URLs below.
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20201023-182805331
Abstract
Metal affinity partitioning has been developed to selectively increase the partition coefficients of metal-binding proteins in aqueous two-phase systems (1). Addition of a small amount of Cu(II)IDA-PEG (Cu(II)-iminodiacetic acid-derivatized polyethyleneglycol) to a two-phase system enhances the partitioning of proteins which contain surface-accessible histidines, due to the metal-coordinating ability of histidine. This technique has also been used as a tool to measure stability constants for the metal-protein complexes formed between metal-binding sites and Cu(II) chelated by IDA-PEG (2).
| Item Type: | Book Section | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Related URLs: |
| |||||||||
| ORCID: |
| |||||||||
| Additional Information: | © Springer-Verlag Tokyo 1992. | |||||||||
| Subject Keywords: | metal affinity partitioning phosphoprotein aqueous two-phase system | |||||||||
| DOI: | 10.1007/978-4-431-68180-9_148 | |||||||||
| Record Number: | CaltechAUTHORS:20201023-182805331 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20201023-182805331 | |||||||||
| Official Citation: | Chung B.H., Arnold F.H. (1992) Metal Affinity Partitioning of Phosphoproteins in Aqueous Two-Phase Systems. In: Furusaki S., Endo I., Matsuno R. (eds) Biochemical Engineering for 2001. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68180-9_148 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 106271 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Rebecca Minjarez | |||||||||
| Deposited On: | 26 Oct 2020 16:00 | |||||||||
| Last Modified: | 16 Nov 2021 18:52 |
Repository Staff Only: item control page
