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Metal Affinity Partitioning of Phosphoproteins in Aqueous Two-Phase Systems

Chung, Bong Hyun and Arnold, Frances H. (1992) Metal Affinity Partitioning of Phosphoproteins in Aqueous Two-Phase Systems. In: Biochemical Engineering for 2001. Springer Japan , Tokyo, pp. 557-559. ISBN 9784431681823. https://resolver.caltech.edu/CaltechAUTHORS:20201023-182805331

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Abstract

Metal affinity partitioning has been developed to selectively increase the partition coefficients of metal-binding proteins in aqueous two-phase systems (1). Addition of a small amount of Cu(II)IDA-PEG (Cu(II)-iminodiacetic acid-derivatized polyethyleneglycol) to a two-phase system enhances the partitioning of proteins which contain surface-accessible histidines, due to the metal-coordinating ability of histidine. This technique has also been used as a tool to measure stability constants for the metal-protein complexes formed between metal-binding sites and Cu(II) chelated by IDA-PEG (2).


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1007/978-4-431-68180-9_148DOIArticle
https://rdcu.be/b9cxEPublisherFree ReadCube access
ORCID:
AuthorORCID
Arnold, Frances H.0000-0002-4027-364X
Additional Information:© Springer-Verlag Tokyo 1992.
Subject Keywords:metal affinity partitioning phosphoprotein aqueous two-phase system
DOI:10.1007/978-4-431-68180-9_148
Record Number:CaltechAUTHORS:20201023-182805331
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201023-182805331
Official Citation:Chung B.H., Arnold F.H. (1992) Metal Affinity Partitioning of Phosphoproteins in Aqueous Two-Phase Systems. In: Furusaki S., Endo I., Matsuno R. (eds) Biochemical Engineering for 2001. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68180-9_148
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:106271
Collection:CaltechAUTHORS
Deposited By: Rebecca Minjarez
Deposited On:26 Oct 2020 16:00
Last Modified:16 Nov 2021 18:52

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