Chung, Bong Hyun and Arnold, Frances H. (1992) Metal Affinity Partitioning of Phosphoproteins in Aqueous Two-Phase Systems. In: Biochemical Engineering for 2001. Springer Japan , Tokyo, pp. 557-559. ISBN 9784431681823. https://resolver.caltech.edu/CaltechAUTHORS:20201023-182805331
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Abstract
Metal affinity partitioning has been developed to selectively increase the partition coefficients of metal-binding proteins in aqueous two-phase systems (1). Addition of a small amount of Cu(II)IDA-PEG (Cu(II)-iminodiacetic acid-derivatized polyethyleneglycol) to a two-phase system enhances the partitioning of proteins which contain surface-accessible histidines, due to the metal-coordinating ability of histidine. This technique has also been used as a tool to measure stability constants for the metal-protein complexes formed between metal-binding sites and Cu(II) chelated by IDA-PEG (2).
Item Type: | Book Section | |||||||||
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Additional Information: | © Springer-Verlag Tokyo 1992. | |||||||||
Subject Keywords: | metal affinity partitioning phosphoprotein aqueous two-phase system | |||||||||
DOI: | 10.1007/978-4-431-68180-9_148 | |||||||||
Record Number: | CaltechAUTHORS:20201023-182805331 | |||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20201023-182805331 | |||||||||
Official Citation: | Chung B.H., Arnold F.H. (1992) Metal Affinity Partitioning of Phosphoproteins in Aqueous Two-Phase Systems. In: Furusaki S., Endo I., Matsuno R. (eds) Biochemical Engineering for 2001. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68180-9_148 | |||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
ID Code: | 106271 | |||||||||
Collection: | CaltechAUTHORS | |||||||||
Deposited By: | Rebecca Minjarez | |||||||||
Deposited On: | 26 Oct 2020 16:00 | |||||||||
Last Modified: | 16 Nov 2021 18:52 |
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