Kim, Taewoo and Serabyn, Eugene and Schadegg, Maximilian and Liewer, Kurt and Oborny, Nathan and Wallace, J. Kent and Rider, Stephanie and Bedrossian, Manuel and Lindensmith, Christian and Nadeau, Jay (2020) ELVIS: A Correlated Light-Field and Digital Holographic Microscope for Field and Laboratory Investigations. Microscopy Today, 28 (3). pp. 18-25. ISSN 1551-9295. doi:10.1017/s1551929520000899. https://resolver.caltech.edu/CaltechAUTHORS:20201109-080655639
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Abstract
This is the first of two articles on the Extant Life Volumetric Imaging System (ELVIS) describing a combined digital holographic microscope (DHM) and a fluorescence light-field microscope (FLFM). The instrument is modular and robust enough for field use. Each mode uses its own illumination source and camera, but both microscopes share a common objective lens and sample viewing chamber. This allows correlative volumetric imaging in amplitude, quantitative phase, and fluorescence modes. A detailed schematic and parts list is presented, as well as links to open-source software packages for data acquisition and analysis that permits interested researchers to duplicate the design. Instrument performance is quantified using test targets and beads. In the second article on ELVIS, to be published in the next issue of Microscopy Today, analysis of data from field tests and images of microorganisms will be presented.
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| Additional Information: | © 2020 Microscopy Society of America. Published online by Cambridge University Press: 18 May 2020. Portions of this work were performed under a contract from the Jet Propulsion Laboratory, California Institute of Technology and at the Jet Propulsion Laboratory, California Institute of Technology under contract with the National Aeronautics and Space Administration. Portions of this work were supported by NSF Award #1828793. | |||||||||
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| Subject Keywords: | holographic microscopy, light-field microscopy, volumetric imaging, lenslet arrays, reconstruction software | |||||||||
| Issue or Number: | 3 | |||||||||
| DOI: | 10.1017/s1551929520000899 | |||||||||
| Record Number: | CaltechAUTHORS:20201109-080655639 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20201109-080655639 | |||||||||
| Official Citation: | Kim, T., Serabyn, E., Schadegg, M., Liewer, K., Oborny, N., Kent Wallace, J., . . . Nadeau, J. (2020). ELVIS: A Correlated Light-Field and Digital Holographic Microscope for Field and Laboratory Investigations. Microscopy Today, 28(3), 18-25. doi:10.1017/S1551929520000899 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 106500 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Tony Diaz | |||||||||
| Deposited On: | 09 Nov 2020 16:19 | |||||||||
| Last Modified: | 16 Nov 2021 18:54 |
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