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Cell-cycle dependent organization and dynamics of RNA Polymerase I in live human cells

Conway, William and Cho, Won-Ki and Jayanth, Namrata and Mullen, Susan and Cissé, Ibrahim I. (2017) Cell-cycle dependent organization and dynamics of RNA Polymerase I in live human cells. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20201112-143949148

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Abstract

RNA Polymerase I (Pol I) is responsible for over 60% of transcriptional output in human cells, yet basic questions concerning the spatial and temporal organization of the polymerase remain unanswered. Here we investigate how mammalian cells rely on Pol I organization throughout the cell cycle to balance different needs, from complete transcription shut down to massive increase in protein synthesis (and thus ribosomal RNA synthesis) before cell division. In contrast to our previous reports on RNA Polymerase II, Pol I clusters are stable with active transcription, and the presence of transient Pol I clusters correlates with inactive ribosomal transcription. Our results suggest that both stable and transient populations Pol I clusters co-exist in individual living cells, and their relative fraction may directly reflect the global gene expression need of the cell.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/133082DOIDiscussion Paper
ORCID:
AuthorORCID
Cho, Won-Ki0000-0001-9336-0686
Cissé, Ibrahim I.0000-0002-8764-1809
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. We would like to thank Cisse Lab members Arjun Narayanan, Takuma Inoue, and Jan-Hendrick Spille (MIT) for helpful comments, and J Owen Andrews for comments and assistance with analysis software. Research reported in this publication was supported by the National Cancer Institute of the National Institutes of Health under the NIH Director’s New Innovator Award (DP2CA195769) to I.I.C. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. This work was also supported by funds from the MIT Department of Physics, the DeFlorez Endowment Fund, and the MIT Undergraduate Research Opportunities Program (UROP).
Funders:
Funding AgencyGrant Number
NIHDP2CA195769
Massachusetts Institute of Technology (MIT)UNSPECIFIED
Record Number:CaltechAUTHORS:20201112-143949148
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201112-143949148
Official Citation:Cell-cycle dependent organization and dynamics of RNA Polymerase I in live human cells William Conway, Won-Ki Cho, Namrata Jayanth, Susan Mullen, Ibrahim I Cissé bioRxiv 133082; doi: https://doi.org/10.1101/133082
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:106656
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:16 Nov 2020 16:40
Last Modified:16 Nov 2020 16:40

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