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BMP signalling is required for extra-embryonic ectoderm development during pre-to-post-implantation transition of the mouse embryo

Sozen, Berna and Demir, Necdet and Zernicka-Goetz, Magdalena (2021) BMP signalling is required for extra-embryonic ectoderm development during pre-to-post-implantation transition of the mouse embryo. Developmental Biology, 470 . pp. 84-94. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20201117-110225184

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Abstract

At implantation, the mouse embryo undergoes a critical transformation which requires the precise spatiotemporal control of signalling pathways necessary for morphogenesis and developmental progression. The role played by such signalling pathways during this transition are largely unexplored, due to the inaccessibility of the embryo during the implantation when it becomes engulfed by uterine tissues. Genetic studies demonstrate that mutant embryos for BMPs die around gastrulation. Here we have aimed to dissect the role of BMPs during pre-to post-implantation transition by using a protocol permitting the development of the embryo beyond implantation stages in vitro and using stem cells to mimic post-implantation tissue organisation. By assessing both the canonical and non-canonical mechanisms of BMP, we show that the loss of canonical BMP activity compromises the extra-embryonic ectoderm development. Our analyses demonstrate that BMP signalling maintains stem cell populations within both embryonic/extra-embryonic tissues during pre-to post-implantation development. These results may provide insight into the role played by BMP signalling in controlling early embryogenesis.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.ydbio.2020.11.005DOIArticle
ORCID:
AuthorORCID
Sozen, Berna0000-0001-5834-5819
Demir, Necdet0000-0002-8166-5535
Zernicka-Goetz, Magdalena0000-0002-7004-2471
Additional Information:© 2020 Published by Elsevier Inc. Received 7 May 2020, Revised 6 November 2020, Accepted 8 November 2020, Available online 17 November 2020. We thank colleagues in the M.Z.G. laboratory for insightful comments. We are grateful to Andy Cox for critical reading of the manuscript and drawing models in Figs. 1A and 2E. The M.Z.G. laboratory is supported by grants from the European Research Council (669198); the Wellcome Trust (098287/Z/12/Z). B.S. is grateful to Yale School of Medicine for start-up funding and also supported by the International Research Fellowship Program 2214/A from Scientific and Technological Research Council of Turkey, and the Akdeniz University Scientific Research Fund (Project No: TDK-2015-1109). Author contributions: Conceptualization: B.S., M.Z-G.; Methodology & Investigation: B.S. Writing: B.S, M.Z-G.; Supervision: N.D., M.Z-G. Funding: B.S., N.D., M.Z.-G. The authors declare no competing or financial interests.
Funders:
Funding AgencyGrant Number
European Research Council (ERC)669198
Wellcome Trust098287/Z/12/Z
Yale School of MedicineUNSPECIFIED
Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK)2214/A
Akdeniz ÜniversitesiTDK-2015-1109
Subject Keywords:BMP; Extraembryonic ectoderm; Trophoblast stem cells; Embryonic stem cells
Record Number:CaltechAUTHORS:20201117-110225184
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201117-110225184
Official Citation:Berna Sozen, Necdet Demir, Magdalena Zernicka-Goetz, BMP signalling is required for extra-embryonic ectoderm development during pre-to-post-implantation transition of the mouse embryo, Developmental Biology, Volume 470, 2021, Pages 84-94, ISSN 0012-1606, https://doi.org/10.1016/j.ydbio.2020.11.005. (http://www.sciencedirect.com/science/article/pii/S0012160620302979)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:106701
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:17 Nov 2020 19:16
Last Modified:02 Dec 2020 23:38

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